Co-existence of tyrosine hydroxylase and calcitonin gene-related peptide in cochlear spiral modiolar artery of guinea pigs

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The distribution of tyrosine hydroxylase (TH) and calcitonin gene-related peptide (CGRP) on the cochlear spiral modiolar artery (SMA) was investigated in the guinea pig. The SMA was dissected from the modiolus so that the entire length of the vessel and many of its branches could be observed. Immunohistochemical labeling and double immunofluorescence were employed to localize each compound and to determine whether the TH and CGRP co-exist in neurons of the SMA. Microscopic examination of whole vessel preparations revealed numerous TH- and CGRP-positive neural networks innervating the SMA and its branches. The labeled neurons showed distinct arborization, varicosities and overlap, and were of different diameters. Confocal immunofluorescence microscopy of double-labeled TH and CGRP neurons showed that a number of the TH- and CGRP-positive neurons were co-labeled. Thus, TH and CGRP partially co-exist within the neuronal innervation of SMA. These findings support a hypothesis that specific neuropeptide and adrenergic neurons regulate cochlear blood flow.

Original languageEnglish (US)
Pages (from-to)152-160
Number of pages9
JournalHearing Research
Volume155
Issue number1-2
DOIs
StatePublished - May 15 2001

Keywords

  • Calcitonin gene-related peptide
  • Cochlea
  • Immunofluorescence
  • Spiral modiolar artery
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Sensory Systems

Fingerprint Dive into the research topics of 'Co-existence of tyrosine hydroxylase and calcitonin gene-related peptide in cochlear spiral modiolar artery of guinea pigs'. Together they form a unique fingerprint.

  • Cite this