Co-cultivation of retinoblastoma with fibroblasts, iris pigment epithelium, and retinal pigment epithelium in tissue culture

M. J. Weiner, Daniel Albert, B. L. Gallie, J. L. Craft

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Retinoblastoma cells of the Y79 line were co-cultivated with human fibroblasts and bovine iris and retinal pigment epithelium in tissue culture. The Y79 cells, wich characteristically grow as a suspension culture, were found to attach directly to the fibroblasts and pigment epithelium on the flask surface. Electron microscopic examination of the fibroblast-retinoblastoma co-cultures revealed numerous pinocytotic vesicles lining the fibroblast cell borders that were in contact with the tumor cells. The retinoblastoma cells contained increased numbers of ribosomes, endoplasmic reticulum, and mitochondria. Fibroblastic processes appeared to wrap around and engulf tumor cells. In both the iris and retinal pigment epithelium co-cultures with retinoblastoma cells, there were increased numbers of mitochondria in the tumor cells in areas adjacent to pigment epithelium but no pinocytotic vesicles were seen. The pigment epithelium attached to Y79 cells showed fewer processes than did the fibroblasts in co-culture. In summary, both fibroblast and pigment epithelium functioned as an effective carrier cell layer for retinoblastoma cells. In addition, we believe that the fibroblast layer removed substances secreted by the tumor cells via pinocytotic vesicles.

Original languageEnglish (US)
Pages (from-to)943-957
Number of pages15
JournalInvestigative Ophthalmology and Visual Science
Volume24
Issue number7
StatePublished - Jan 1 1983
Externally publishedYes

Fingerprint

Retinoblastoma
Retinal Pigment Epithelium
Iris
Fibroblasts
Epithelium
Coculture Techniques
Neoplasms
Mitochondria
Ribosomes
Endoplasmic Reticulum
Suspensions
Electrons
Cell Line

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Co-cultivation of retinoblastoma with fibroblasts, iris pigment epithelium, and retinal pigment epithelium in tissue culture. / Weiner, M. J.; Albert, Daniel; Gallie, B. L.; Craft, J. L.

In: Investigative Ophthalmology and Visual Science, Vol. 24, No. 7, 01.01.1983, p. 943-957.

Research output: Contribution to journalArticle

@article{0967dfe60654481eb7a3f4463133b0e2,
title = "Co-cultivation of retinoblastoma with fibroblasts, iris pigment epithelium, and retinal pigment epithelium in tissue culture",
abstract = "Retinoblastoma cells of the Y79 line were co-cultivated with human fibroblasts and bovine iris and retinal pigment epithelium in tissue culture. The Y79 cells, wich characteristically grow as a suspension culture, were found to attach directly to the fibroblasts and pigment epithelium on the flask surface. Electron microscopic examination of the fibroblast-retinoblastoma co-cultures revealed numerous pinocytotic vesicles lining the fibroblast cell borders that were in contact with the tumor cells. The retinoblastoma cells contained increased numbers of ribosomes, endoplasmic reticulum, and mitochondria. Fibroblastic processes appeared to wrap around and engulf tumor cells. In both the iris and retinal pigment epithelium co-cultures with retinoblastoma cells, there were increased numbers of mitochondria in the tumor cells in areas adjacent to pigment epithelium but no pinocytotic vesicles were seen. The pigment epithelium attached to Y79 cells showed fewer processes than did the fibroblasts in co-culture. In summary, both fibroblast and pigment epithelium functioned as an effective carrier cell layer for retinoblastoma cells. In addition, we believe that the fibroblast layer removed substances secreted by the tumor cells via pinocytotic vesicles.",
author = "Weiner, {M. J.} and Daniel Albert and Gallie, {B. L.} and Craft, {J. L.}",
year = "1983",
month = "1",
day = "1",
language = "English (US)",
volume = "24",
pages = "943--957",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "7",

}

TY - JOUR

T1 - Co-cultivation of retinoblastoma with fibroblasts, iris pigment epithelium, and retinal pigment epithelium in tissue culture

AU - Weiner, M. J.

AU - Albert, Daniel

AU - Gallie, B. L.

AU - Craft, J. L.

PY - 1983/1/1

Y1 - 1983/1/1

N2 - Retinoblastoma cells of the Y79 line were co-cultivated with human fibroblasts and bovine iris and retinal pigment epithelium in tissue culture. The Y79 cells, wich characteristically grow as a suspension culture, were found to attach directly to the fibroblasts and pigment epithelium on the flask surface. Electron microscopic examination of the fibroblast-retinoblastoma co-cultures revealed numerous pinocytotic vesicles lining the fibroblast cell borders that were in contact with the tumor cells. The retinoblastoma cells contained increased numbers of ribosomes, endoplasmic reticulum, and mitochondria. Fibroblastic processes appeared to wrap around and engulf tumor cells. In both the iris and retinal pigment epithelium co-cultures with retinoblastoma cells, there were increased numbers of mitochondria in the tumor cells in areas adjacent to pigment epithelium but no pinocytotic vesicles were seen. The pigment epithelium attached to Y79 cells showed fewer processes than did the fibroblasts in co-culture. In summary, both fibroblast and pigment epithelium functioned as an effective carrier cell layer for retinoblastoma cells. In addition, we believe that the fibroblast layer removed substances secreted by the tumor cells via pinocytotic vesicles.

AB - Retinoblastoma cells of the Y79 line were co-cultivated with human fibroblasts and bovine iris and retinal pigment epithelium in tissue culture. The Y79 cells, wich characteristically grow as a suspension culture, were found to attach directly to the fibroblasts and pigment epithelium on the flask surface. Electron microscopic examination of the fibroblast-retinoblastoma co-cultures revealed numerous pinocytotic vesicles lining the fibroblast cell borders that were in contact with the tumor cells. The retinoblastoma cells contained increased numbers of ribosomes, endoplasmic reticulum, and mitochondria. Fibroblastic processes appeared to wrap around and engulf tumor cells. In both the iris and retinal pigment epithelium co-cultures with retinoblastoma cells, there were increased numbers of mitochondria in the tumor cells in areas adjacent to pigment epithelium but no pinocytotic vesicles were seen. The pigment epithelium attached to Y79 cells showed fewer processes than did the fibroblasts in co-culture. In summary, both fibroblast and pigment epithelium functioned as an effective carrier cell layer for retinoblastoma cells. In addition, we believe that the fibroblast layer removed substances secreted by the tumor cells via pinocytotic vesicles.

UR - http://www.scopus.com/inward/record.url?scp=0020512644&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020512644&partnerID=8YFLogxK

M3 - Article

VL - 24

SP - 943

EP - 957

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 7

ER -