Clusters of nontuberculous mycobacteria linked to water sources at three Veterans Affairs medical centers

Gina Oda, Mark A. Winters, Susan M. Pacheco, Monica K. Sikka, Susan C. Bleasdale, Bruce Dunn, Erin Boswell, Benjamin Winters, Amelia Bumsted, Jennifer Frisch, Mark Holodniy

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Objective: To characterize nontuberculous mycobacteria (NTM) associated with case clusters at 3 medical facilities.Design: Retrospective cohort study using molecular typing of patient and water isolates.Setting: Veterans Affairs Medical Centers (VAMCs).Methods: Isolation and identification of NTM from clinical and water samples using culture, MALDI-TOF, and gene population sequencing to determine species and genetic relatedness. Clinical data were abstracted from electronic health records.Results: An identical strain of Mycobacterium conceptionense was isolated from 41 patients at VA Medical Centers (VAMCs A, B, and D), and from VAMC A's ICU ice machine. Isolates were initially identified as other NTM species within the M. fortuitum clade. Sequencing analyses revealed that they were identical M. conceptionense strains. Overall, 7 patients (17%) met the criteria for pulmonary or nonpulmonary infection with NTM, and 13 of 41 (32%) were treated with effective antimicrobials regardless of infection or colonization status. Separately, a M. mucogenicum patient strain from VAMC A matched a strain isolated from a VAMC B ICU ice machine. VAMC C, in a different state, had a 4-patient cluster with Mycobacterium porcinum. Strains were identical to those isolated from sink-water samples at this facility.Conclusion: NTM from hospital water systems are found in hospitalized patients, often during workup for other infections, making attribution of NTM infection problematic. Variable NTM identification methods and changing taxonomy create challenges for epidemiologic investigation and linkage to environmental sources.

Original languageEnglish (US)
Pages (from-to)320-330
Number of pages11
JournalInfection control and hospital epidemiology
Volume41
Issue number3
DOIs
StatePublished - Mar 1 2020
Externally publishedYes

ASJC Scopus subject areas

  • Epidemiology
  • Microbiology (medical)
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Clusters of nontuberculous mycobacteria linked to water sources at three Veterans Affairs medical centers'. Together they form a unique fingerprint.

Cite this