Cloning the arterial IgA antibody response during acute Kawasaki disease

Anne H. Rowley, Stanford T. Shulman, Francesca L. Garcia, Judith Guzman-Cottrill, Masaru Miura, Hannah L. Lee, Susan C. Baker

Research output: Contribution to journalArticle

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Abstract

Kawasaki disease (KD) is the most common acquired cardiac disease in children in developed nations. The etiology of KD is unknown but likely to be a ubiquitous microbial agent. Previously, we showed that oligoclonal IgA plasma cells infiltrate coronary arteries and other inflamed tissues in acute KD. We demonstrated that a synthetic Ab made using an α H chain sequence prevalent in acute KD arterial tissue detected Ag in acute KD coronary arteries, lung, and other inflamed tissues and that Ag localized to cytoplasmic inclusion bodies in the acute KD ciliated bronchial epithelium. In this study, we synthesized a panel of mAbs from α and κ chain sequences present in the KD arterial wall and tested the Abs for binding to acute KD tissues. We report that all of the synthetic mAbs that bind to acute KD tissues detect Ag in cytoplasmic inclusion bodies in the acute KD ciliated bronchial epithelium. Abs made from α sequences that were prevalent in KD arterial tissue show stronger binding to acute KD tissues than Abs made from less prevalent sequences. These findings highlight the likely importance of the inclusion bodies in the etiopathogenesis of acute KD, confirm that the IgA Ab response in acute KD is Ag driven, and demonstrate the usefulness of cloning the Ab response in diseased tissues to identify disease-relevant Ags.

Original languageEnglish (US)
Pages (from-to)8386-8391
Number of pages6
JournalJournal of Immunology
Volume175
Issue number12
StatePublished - Dec 15 2005
Externally publishedYes

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Mucocutaneous Lymph Node Syndrome
Acute Disease
Immunoglobulin A
Antibody Formation
Organism Cloning
Inclusion Bodies
Coronary Vessels
Epithelium
Plasma Cells
Developed Countries

ASJC Scopus subject areas

  • Immunology

Cite this

Rowley, A. H., Shulman, S. T., Garcia, F. L., Guzman-Cottrill, J., Miura, M., Lee, H. L., & Baker, S. C. (2005). Cloning the arterial IgA antibody response during acute Kawasaki disease. Journal of Immunology, 175(12), 8386-8391.

Cloning the arterial IgA antibody response during acute Kawasaki disease. / Rowley, Anne H.; Shulman, Stanford T.; Garcia, Francesca L.; Guzman-Cottrill, Judith; Miura, Masaru; Lee, Hannah L.; Baker, Susan C.

In: Journal of Immunology, Vol. 175, No. 12, 15.12.2005, p. 8386-8391.

Research output: Contribution to journalArticle

Rowley, AH, Shulman, ST, Garcia, FL, Guzman-Cottrill, J, Miura, M, Lee, HL & Baker, SC 2005, 'Cloning the arterial IgA antibody response during acute Kawasaki disease', Journal of Immunology, vol. 175, no. 12, pp. 8386-8391.
Rowley AH, Shulman ST, Garcia FL, Guzman-Cottrill J, Miura M, Lee HL et al. Cloning the arterial IgA antibody response during acute Kawasaki disease. Journal of Immunology. 2005 Dec 15;175(12):8386-8391.
Rowley, Anne H. ; Shulman, Stanford T. ; Garcia, Francesca L. ; Guzman-Cottrill, Judith ; Miura, Masaru ; Lee, Hannah L. ; Baker, Susan C. / Cloning the arterial IgA antibody response during acute Kawasaki disease. In: Journal of Immunology. 2005 ; Vol. 175, No. 12. pp. 8386-8391.
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