Cloning Primates

Calvin Simerly, Masahito Tachibana, Shoukhrat Mitalipov, Gerald Schatten

    Research output: Chapter in Book/Report/Conference proceedingChapter

    Abstract

    The promise of nuclear transfer (NT) in producing patient-specific embryonic stem cells for regenerative medicine holds great interest for the treatment of human diseases, whether as tools for "disease in a dish" discoveries or as replacement cells in patient therapies. With the discovery of induced pluripotent stem cells, current debates flourish over whether human NT is necessary, given the limitations of burdensome technical, ethical, and legal issues. Non-human primates (NHP) are superior models for understanding the stepwise events of successful primate NT and could provide easier extrapolations to events in humans than rodent cloning model. Early challenges to producing cloned NHP blastocysts using traditional NT technologies successful in rodent and cows were overcome by modifications to enucleation, artificial activation, and embryo culture in monkeys. Regardless, adoption of these technical advances in human NT has yet to produce stable human pluripotent embryonic stem cells, and recent reports suggest that human meiotic spindle removal as the first step in the cloning process remains detrimental to producing viable human ESC lines. This chapter provides a review on challenges in nuclear transfer in primates - non-human and human alike.

    Original languageEnglish (US)
    Title of host publicationPrinciples of Cloning
    Subtitle of host publicationSecond Edition
    PublisherElsevier Inc.
    Pages299-310
    Number of pages12
    ISBN (Print)9780123865410
    DOIs
    StatePublished - Oct 1 2013

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    Keywords

    • Centriole
    • Centrosomes
    • Eg5
    • HSET
    • Microtubules
    • Molecular motors
    • NuMA
    • Nuclear transfer
    • Primate cloning
    • Regenerative medicine
    • Somatic cell nuclear transfer
    • Spindle enucleation
    • Stem cell

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Simerly, C., Tachibana, M., Mitalipov, S., & Schatten, G. (2013). Cloning Primates. In Principles of Cloning: Second Edition (pp. 299-310). Elsevier Inc.. https://doi.org/10.1016/B978-0-12-386541-0.00023-0