Cloning of the cDNA and gene for a human D2 dopamine receptor

D. K. Grandy; M. A. Marchionni; H. Makam; R. E. Stofko; M. Alfano; L. Frothingham; J. B. Fischer; K. J. Burke-Howie; J. R. Bunzow; A. C. Server; O. Civelli

doi:10.1073/pnas.86.24.9762

Cloning of the cDNA and gene for a human D₂ dopamine receptor

D. K. Grandy, M. A. Marchionni, H. Makam, R. E. Stofko, M. Alfano, L. Frothingham, J. B. Fischer, K. J. Burke-Howie, J. R. Bunzow, A. C. Server, O. Civelli

Research output: Contribution to journal › Article › peer-review

513 Scopus citations

Abstract

A clone encoding a human D₂ dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D₂ receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

Original language	English (US)
Pages (from-to)	9762-9766
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	86
Issue number	24
DOIs	https://doi.org/10.1073/pnas.86.24.9762
State	Published - 1989

Keywords

DRD2
Schizophrenia
alternative splicing
introns
receptor evolution

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.86.24.9762

Cite this

Grandy, D. K., Marchionni, M. A., Makam, H., Stofko, R. E., Alfano, M., Frothingham, L., Fischer, J. B., Burke-Howie, K. J., Bunzow, J. R., Server, A. C., & Civelli, O. (1989). Cloning of the cDNA and gene for a human D₂ dopamine receptor. Proceedings of the National Academy of Sciences of the United States of America, 86(24), 9762-9766. https://doi.org/10.1073/pnas.86.24.9762

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abstract = "A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.",

keywords = "DRD2, Schizophrenia, alternative splicing, introns, receptor evolution",

author = "Grandy, {D. K.} and Marchionni, {M. A.} and H. Makam and Stofko, {R. E.} and M. Alfano and L. Frothingham and Fischer, {J. B.} and Burke-Howie, {K. J.} and Bunzow, {J. R.} and Server, {A. C.} and O. Civelli",

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T1 - Cloning of the cDNA and gene for a human D2 dopamine receptor

AU - Grandy, D. K.

AU - Marchionni, M. A.

AU - Makam, H.

AU - Stofko, R. E.

AU - Alfano, M.

AU - Frothingham, L.

AU - Fischer, J. B.

AU - Burke-Howie, K. J.

AU - Bunzow, J. R.

AU - Server, A. C.

AU - Civelli, O.

PY - 1989

Y1 - 1989

N2 - A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

AB - A clone encoding a human D2 dopamine receptor was isolated from a pituitary cDNA library and sequenced. The deduced protein sequence is 96% identical with that of the cloned rat receptor with one major difference: the human receptor contains an additional 29 amino acids in its putative third cytoplasmic loop. Southern blotting demonstrated the presence of only one human D2 receptor gene. Two overlapping phage containing the gene were isolated and characterized. DNA sequence analysis of these clones showed that the coding sequence is interrupted by six introns and that the additional amino acids present in the human pituitary receptor are encoded by a single exon of 87 base pairs. The involvement of this sequence in alternative splicing and its biological significance are discussed.

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KW - Schizophrenia

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KW - introns

KW - receptor evolution

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