Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting

C. R. Müller, K. E. Davies, C. Cremer, G. Rappold, J. W. Gray, H. H. Ropers

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Human Y chromosomes were purified by dual beam flow sorting from a human x Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into λgtWES.λB vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalHuman genetics
Volume64
Issue number2
DOIs
StatePublished - Aug 1 1983

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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