Abstract
Human Y chromosomes were purified by dual beam flow sorting from a human x Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into λgtWES.λB vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.
Original language | English (US) |
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Pages (from-to) | 110-115 |
Number of pages | 6 |
Journal | Human genetics |
Volume | 64 |
Issue number | 2 |
DOIs | |
State | Published - Aug 1983 |
Externally published | Yes |
ASJC Scopus subject areas
- Genetics
- Genetics(clinical)