Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting

C. R. Müller, K. E. Davies, C. Cremer, G. Rappold, Joe Gray, H. H. Ropers

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Human Y chromosomes were purified by dual beam flow sorting from a human x Chinese hamster cell line retaining the Y as the only free human chromosome. DNA was extracted from the Y fraction and cloned into λgtWES.λB vector arms. More than 100 recombinant clones carrying human inserts have been characterised by Benton-Davis plaque screening and Southern blotting or in situ hybridisation. Several repetitive sequences were found to be predominantly located on the Y, whereas the majority also cross-hybridised with autosomal DNA. One repetitive clone gave a specific hybridisation signal with the X and the Y chromosome but not with autosomes. Preliminary evidence indicates that many clones contain single copy as well as repetitive sequences. However, no Y-specific single copy sequence has yet been identified.

Original languageEnglish (US)
Pages (from-to)110-115
Number of pages6
JournalHuman Genetics
Volume64
Issue number2
DOIs
StatePublished - Aug 1983
Externally publishedYes

Fingerprint

Chromosomes, Human, Y
Organism Cloning
Clone Cells
Nucleic Acid Repetitive Sequences
Y Chromosome
DNA
Human Chromosomes
X Chromosome
Southern Blotting
Cricetulus
In Situ Hybridization
Cell Line

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting. / Müller, C. R.; Davies, K. E.; Cremer, C.; Rappold, G.; Gray, Joe; Ropers, H. H.

In: Human Genetics, Vol. 64, No. 2, 08.1983, p. 110-115.

Research output: Contribution to journalArticle

Müller, C. R. ; Davies, K. E. ; Cremer, C. ; Rappold, G. ; Gray, Joe ; Ropers, H. H. / Cloning of genomic sequences from the human Y chromosome after purification by dual beam flow sorting. In: Human Genetics. 1983 ; Vol. 64, No. 2. pp. 110-115.
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