Cloning of decidual prolactin from rhesus macaque

N. A. Brown, Cynthia Bethea

    Research output: Contribution to journalArticle

    14 Citations (Scopus)

    Abstract

    Rhesus monkey (Macaca mulatta) decidual prolactin cDNA was cloned and sequenced. The first 210 bp of the monkey sequence is not contiguous to the human pituitary prolactin transcription initiation site. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern analysis confirmed that this 5'-extended prolactin transcript is present in monkey decidua but not in pituitary. Isolation and sequencing of a partial genomic monkey prolactin clone verified the nucleotide changes found in the rhesus cDNA. Comparison of the rhesus decidual prolactin cDNA to the human pituitary cDNA revealed more than 97% homology between the two. These data indicate that monkey prolactin mRNA from the decidualized endometrium of pregnancy contains unique 5' sequence not present in pituitary prolactin mRNA, suggesting the use of an alternative start site. Thus it is likely that the decidua uses promoter and regulatory regions different from those reported for pituitary prolactin.

    Original languageEnglish (US)
    Pages (from-to)543-552
    Number of pages10
    JournalBiology of Reproduction
    Volume50
    Issue number3
    StatePublished - 1994

    Fingerprint

    Macaca mulatta
    Prolactin
    Organism Cloning
    Haplorhini
    Complementary DNA
    Decidua
    Messenger RNA
    Nucleic Acid Regulatory Sequences
    Transcription Initiation Site
    Endometrium
    Genetic Promoter Regions
    Reverse Transcription
    Nucleotides
    Clone Cells
    Pregnancy
    Polymerase Chain Reaction

    ASJC Scopus subject areas

    • Cell Biology
    • Developmental Biology
    • Embryology

    Cite this

    Cloning of decidual prolactin from rhesus macaque. / Brown, N. A.; Bethea, Cynthia.

    In: Biology of Reproduction, Vol. 50, No. 3, 1994, p. 543-552.

    Research output: Contribution to journalArticle

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