Cloning of a Novel Rat Kidney cDNA Homologous to CHIP28 and WCH-CD Water Channels

T. H. Ma, A. Frigeri, William Skach, A. S. Verkman

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Two kidney water channels have been identified: CHIP28 in proximal tubule and thin descending limb, and WCH-CD in collecting duct apical membrane. An homologous cDNA (WCH3) was obtained from rat kidney and found to encode a 276 amino acid, 29 kDa protein with 39% amino acid identity to rat CHIP28, 50% to WCH-CD and 49% to MIP26. The WCH3 transcript of 2.5 kb was expressed exclusively in kidney and was upregulated in dehydrated rats. Cell-free translation produced an ∼28 kDa protein. Analysis of the predicted amino acid sequence indicated a hydrophobic protein with 4-6 membrane-spanning domains, with one N-linked glycosylation site, two conserved NPA boxes common to MIP26 family proteins, and conserved residue C189 common to water channels. WCH3 is a new member of the MIP26 family of channel-forming proteins in mammalian kidney.

Original languageEnglish (US)
Pages (from-to)654-659
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume197
Issue number2
DOIs
StatePublished - Dec 15 1993
Externally publishedYes

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Aquaporins
Cloning
Rats
Organism Cloning
Complementary DNA
Kidney
Proteins
Amino Acids
Glycosylation
Membranes
Protein Sequence Analysis
Ducts
Extremities

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Cloning of a Novel Rat Kidney cDNA Homologous to CHIP28 and WCH-CD Water Channels. / Ma, T. H.; Frigeri, A.; Skach, William; Verkman, A. S.

In: Biochemical and Biophysical Research Communications, Vol. 197, No. 2, 15.12.1993, p. 654-659.

Research output: Contribution to journalArticle

Ma, T. H. ; Frigeri, A. ; Skach, William ; Verkman, A. S. / Cloning of a Novel Rat Kidney cDNA Homologous to CHIP28 and WCH-CD Water Channels. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 197, No. 2. pp. 654-659.
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