Cloning of a new FRTL5-derived cell-line stably expressing active human thyroid peroxidase

Marina Marinovich; Marina Guizzetti; Tiziano Zanelli; Emanuela Corsini; Corrado L. Galli

doi:10.1006/bbrc.1995.2012

Cloning of a new FRTL5-derived cell-line stably expressing active human thyroid peroxidase

Marina Marinovich, Marina Guizzetti, Tiziano Zanelli, Emanuela Corsini, Corrado L. Galli

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 μM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 μM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 μM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.

Original language	English (US)
Pages (from-to)	602-608
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	212
Issue number	2
DOIs	https://doi.org/10.1006/bbrc.1995.2012
State	Published - Jul 17 1995
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1995.2012

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abstract = "A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 μM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 μM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 μM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.",

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AU - Guizzetti, Marina

AU - Zanelli, Tiziano

AU - Corsini, Emanuela

AU - Galli, Corrado L.

PY - 1995/7/17

Y1 - 1995/7/17

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AB - A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 μM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 μM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 μM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.

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Cloning of a new FRTL5-derived cell-line stably expressing active human thyroid peroxidase

Abstract

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