Cloning of a new FRTL5-derived cell-line stably expressing active human thyroid peroxidase

Marina Marinovich, Marina Guizzetti, Tiziano Zanelli, Emanuela Corsini, Corrado L. Galli

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A full-length cDNA clone for human thyroid peroxidase inserted into the mammalian cell expression vector pECE was stably transfected into the rat thyroid cell line FRTL5. Clones expressed immunologically and enzymatically assessed human thyroid peroxidase protein. Methimazole (25 μM) inhibited the thyroid peroxidase activity dose-dependently and this effect was completely antagonised by 100 μM NaI. Ethylenethiourea, metabolite of dithiocarbamate pesticides, inhibited the enzyme at 50 μM. Thus, we have obtained thyroidal cells stably expressing enzymatically active human thyroid peroxidase which can be pharmacologically modulated and studied.

Original languageEnglish (US)
Pages (from-to)602-608
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume212
Issue number2
DOIs
StatePublished - Jul 17 1995

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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