Cloning of a gene from streptomyces species complementing argG mutations

Hiroshi Ishihara; Michiko M. Nakano; Hiroshi Ogawara

doi:10.7164/antibiotics.38.787

Cloning of a gene from streptomyces species complementing argG mutations

Hiroshi Ishihara, Michiko M. Nakano, Hiroshi Ogawara

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 com-plemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG⁺ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.

Original language	English (US)
Pages (from-to)	787-794
Number of pages	8
Journal	the journal of antibiotics
Volume	38
Issue number	6
DOIs	https://doi.org/10.7164/antibiotics.38.787
State	Published - 1985
Externally published	Yes

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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abstract = "A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 com-plemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.",

author = "Hiroshi Ishihara and Nakano, {Michiko M.} and Hiroshi Ogawara",

year = "1985",

doi = "10.7164/antibiotics.38.787",

language = "English (US)",

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pages = "787--794",

journal = "the journal of antibiotics",

issn = "0021-8820",

publisher = "Japan Antibiotics Research Association",

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T1 - Cloning of a gene from streptomyces species complementing argG mutations

AU - Ishihara, Hiroshi

AU - Nakano, Michiko M.

AU - Ogawara, Hiroshi

PY - 1985

Y1 - 1985

N2 - A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 com-plemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.

AB - A gene that complements argG mutations was cloned from Streptomyces coelicolor and Streptomyces lividans by using pIJ702 as a vector. The recombinant plasmid pMCP25 com-plemented argG mutations of S. lividans and S. coelicolor. The inserted DNA of pMCP25 was able to hybridize with argG+ strains of S. lividans, S. coelicolor, S. lavendulae and S. alboniger but not with argG mutants of these strains.

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JO - the journal of antibiotics

JF - the journal of antibiotics

IS - 6

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Cloning of a gene from streptomyces species complementing argG mutations

Abstract

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