Cloning, expression, purification, and preliminary characterization of single-crystal X-ray diffraction of glucosyltransferase B of streptococcus mutans

Joshua L. Mieher, Norbert Schormann, Manisha Patel, Hui Wu, Champion Deivanayagam

Research output: Contribution to journalArticlepeer-review

Abstract

Dental caries characterized by acid damage of tooth enamel is a persistent disease that begins with the formation of biofilms on the tooth surface. The secreted glucosyltransferases enable Streptococcus mutans to synthesize extracellular glucan polymers using ingested starch within the oral cavity, which eventually results in the production of acid, a contributing factor to cariogenesis. In this paper, we report the cloning, expression, purification, crystallization, and preliminary X-ray diffraction characterization of glucosyltransferase B.

Original languageEnglish (US)
JournalNatural Product Communications
Volume14
Issue number5
DOIs
StatePublished - May 2019
Externally publishedYes

Keywords

  • Biofilm
  • Dental caries
  • Glucans
  • Glucosyltransferase

ASJC Scopus subject areas

  • Pharmacology
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

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