Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain

Khaled M. Houamed, Joseph L. Kuijper, Teresa L. Gilbert, Betty A. Haldeman, Patrick J. O'Hara, Elleen R. Mulvihill, Wolfhard Almers, Frederick S. Hagen

Research output: Contribution to journalArticle

438 Citations (Scopus)

Abstract

A complementary DNA encoding a G protein-coupled glutamate receptor from rat brain, GluGR, was cloned by functional expression in Xenopus oocytes. The complementary DNA encodes a protein of 1199 amino acids containing a seven-transmembrane motif, flanked by large amino- and carboxyl-terminal domains. This receptor lacks any amino acid sequence similarity with other G protein-coupled receptors, suggesting that it may be a member of a new subfamily. The presence of two introns flanking the central core suggests that GluGR may have evolved by exon shuffling. Expressed in oocytes, GluGR is activated by quisqualate > glutamate > ibotenate > trans-1-aminocyclopentyl-1,3-dicarboxylate, and it is inhibited by 2-amino-3-phosphonopropionate. Activation is blocked by Bordella pertussis toxin. These properties are typical of some metabotropic glutamate receptors.

Original languageEnglish (US)
Pages (from-to)1318-1321
Number of pages4
JournalScience
Volume252
Issue number5010
StatePublished - May 31 1991
Externally publishedYes

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Glutamate Receptors
G-Protein-Coupled Receptors
Oocytes
Organism Cloning
Complementary DNA
Quisqualic Acid
Gene Expression
Metabotropic Glutamate Receptors
Pertussis Toxin
Brain
Xenopus
Introns
Glutamic Acid
Amino Acid Sequence
Exons
Amino Acids
Proteins
2-amino-3-phosphonopropionic acid
1-amino-1,3-dicarboxycyclopentane

ASJC Scopus subject areas

  • General

Cite this

Houamed, K. M., Kuijper, J. L., Gilbert, T. L., Haldeman, B. A., O'Hara, P. J., Mulvihill, E. R., ... Hagen, F. S. (1991). Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain. Science, 252(5010), 1318-1321.

Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain. / Houamed, Khaled M.; Kuijper, Joseph L.; Gilbert, Teresa L.; Haldeman, Betty A.; O'Hara, Patrick J.; Mulvihill, Elleen R.; Almers, Wolfhard; Hagen, Frederick S.

In: Science, Vol. 252, No. 5010, 31.05.1991, p. 1318-1321.

Research output: Contribution to journalArticle

Houamed, KM, Kuijper, JL, Gilbert, TL, Haldeman, BA, O'Hara, PJ, Mulvihill, ER, Almers, W & Hagen, FS 1991, 'Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain', Science, vol. 252, no. 5010, pp. 1318-1321.
Houamed KM, Kuijper JL, Gilbert TL, Haldeman BA, O'Hara PJ, Mulvihill ER et al. Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain. Science. 1991 May 31;252(5010):1318-1321.
Houamed, Khaled M. ; Kuijper, Joseph L. ; Gilbert, Teresa L. ; Haldeman, Betty A. ; O'Hara, Patrick J. ; Mulvihill, Elleen R. ; Almers, Wolfhard ; Hagen, Frederick S. / Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain. In: Science. 1991 ; Vol. 252, No. 5010. pp. 1318-1321.
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