Cloning, expression, and gene structure of a g protein-coupled glutamate receptor from rat brain

Khaled M. Houamed, Joseph L. Kuijper, Teresa L. Gilbert, Betty A. Haldeman, Parick J. O'Hara, Eileen R. Mulvihill, Wolfhard Almers, Frederick S. Hagen

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Abstract

A complementary DNA encoding a G protein-coupled glutamate receptor from rat brain, GluGR, was cloned by functional expression in Xenopus oocytes. The complementary DNA encodes a protein of 1199 amino acids containing a seven-transmembrane motif; flanked by large amino- and carboxyl-terminal domains. This receptor lacks any amino acid sequence similarity with other G protein-coupled receptors, suggesting that it may be a member of a new subfamily. The presence of two introns flanking the central core suggests that GluGR may have evolved by exon shuffling. Expressed in oocytes, GluGR is activated by quisqualate > glutamate > ibotenate > trans-1-aminocyclopentyl-1,3-dicarboxylate, and it is inhibited by 2-amino-3-phosphonopropionate. Activation is blocked by Bordella pertussis toxin. These properties are typical of some metabotropic glutamate receptors.

Original languageEnglish (US)
Pages (from-to)1318-1321
Number of pages4
JournalScience
Volume252
Issue number5010
DOIs
StatePublished - Jan 1 1991

ASJC Scopus subject areas

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    Houamed, K. M., Kuijper, J. L., Gilbert, T. L., Haldeman, B. A., O'Hara, P. J., Mulvihill, E. R., Almers, W., & Hagen, F. S. (1991). Cloning, expression, and gene structure of a g protein-coupled glutamate receptor from rat brain. Science, 252(5010), 1318-1321. https://doi.org/10.1126/science.1656524