Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4)

Yang Cao, Elie Traer, Guy A. Zimmerman, Thomas M. McIntyre, Stephen M. Prescott

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Long-chain fatty acid-CoA ligase (also called fatty acid acyl-CoA synthetase) plays an essential role in lipid biosynthesis and fatty acid degradation. We report herein the cDNA cloning of the human long-chain fatty acid-CoA ligase 4 from a brain library. The cDNA encodes a functional long- chain fatty acid-CoA ligase that shows preference for arachidonic acid as substrate. We also studied the tissue distribution of gene expression by Northern hybridization. Human placenta, brain, testis, ovary, spleen, and adrenal cortex have the highest levels of expression of the long-chain fatty acid-CoA ligase 4, whereas the GI system has the lowest. Finally, this gene was localized to chromosome Xq23 in human by FISH analysis.

Original languageEnglish (US)
Pages (from-to)327-330
Number of pages4
JournalGenomics
Volume49
Issue number2
DOIs
StatePublished - Apr 15 1998
Externally publishedYes

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Organism Cloning
Fatty Acids
Complementary DNA
Coenzyme A Ligases
Acyl Coenzyme A
Adrenal Cortex
Brain
Tissue Distribution
Arachidonic Acid
Placenta
Testis
Ovary
Spleen
Chromosomes
Lipids
Gene Expression
long-chain-fatty-acid-CoA ligase
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4). / Cao, Yang; Traer, Elie; Zimmerman, Guy A.; McIntyre, Thomas M.; Prescott, Stephen M.

In: Genomics, Vol. 49, No. 2, 15.04.1998, p. 327-330.

Research output: Contribution to journalArticle

Cao, Yang ; Traer, Elie ; Zimmerman, Guy A. ; McIntyre, Thomas M. ; Prescott, Stephen M. / Cloning, expression, and chromosomal localization of human long-chain fatty acid-CoA ligase 4 (FACL4). In: Genomics. 1998 ; Vol. 49, No. 2. pp. 327-330.
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