Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11

A. Jardim, S. Hanson, Buddy Ullman, W. D. McCubbin, C. M. Kay, R. W. Olafson

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

This report describes the complete translated gene sequence, predicted secondary structure and lipid bilayer association of a novel kinetoplastid membrane protein (KMP-11) from Leishmania donovani promastigotes. KMP-11 was previously referred to as the lipophosphoglycan-associated protein (LPGAP). The isolation, species distribution and chemical characterization, including a partial protein sequence analysis and post-translational modifications, of this major membrane component have been described. C.d. measurements of KMP-11 indicated a very high helical content estimated to be approximately 86 % in trifluoroethanol. This was in agreement with computer-based secondary-structure analyses which predicted KMP-11 to be almost exclusively α-helical, with the protein adopting a helix-loop-helix motif. Arrangement of the residues located in the putative helical regions on an Edmundson helical wheel showed that this molecule could have a strongly amphipathic conformation and provided an explanation for how such a highly charged protein might be inserted into the plasma membrane. Evidence in support of KMP-11 association with lipid bilayers was provided by showing that KMP-11 could mediate carboxyfluorescein release from liposomes. These findings suggested that KMP-11 may function in part to increase bilayer pressure, stabilizing molecules such as lipophosphoglycan within the parasite pellicular membrane.

Original languageEnglish (US)
Pages (from-to)315-320
Number of pages6
JournalBiochemical Journal
Volume305
Issue number1
StatePublished - 1995
Externally publishedYes

Fingerprint

Cloning
Organism Cloning
Lipid Bilayers
Lipid bilayers
Helix-Loop-Helix Motifs
Trifluoroethanol
Proteins
Membranes
Protein Sequence Analysis
Association reactions
Post Translational Protein Processing
Liposomes
Molecules
Parasites
Cell membranes
Cell Membrane
Conformations
Pressure
Wheels
Membrane Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Jardim, A., Hanson, S., Ullman, B., McCubbin, W. D., Kay, C. M., & Olafson, R. W. (1995). Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11. Biochemical Journal, 305(1), 315-320.

Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11. / Jardim, A.; Hanson, S.; Ullman, Buddy; McCubbin, W. D.; Kay, C. M.; Olafson, R. W.

In: Biochemical Journal, Vol. 305, No. 1, 1995, p. 315-320.

Research output: Contribution to journalArticle

Jardim, A, Hanson, S, Ullman, B, McCubbin, WD, Kay, CM & Olafson, RW 1995, 'Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11', Biochemical Journal, vol. 305, no. 1, pp. 315-320.
Jardim A, Hanson S, Ullman B, McCubbin WD, Kay CM, Olafson RW. Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11. Biochemical Journal. 1995;305(1):315-320.
Jardim, A. ; Hanson, S. ; Ullman, Buddy ; McCubbin, W. D. ; Kay, C. M. ; Olafson, R. W. / Cloning and structure-function analysis of the Leishmania donovani kinetoplastid membrane protein-11. In: Biochemical Journal. 1995 ; Vol. 305, No. 1. pp. 315-320.
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