Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase

C. David Sifri; Keith Wilson; Sarah Smolik; Michael Forte; Buddy Ullman

doi:10.1016/0167-4781(94)90132-5

Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase

C. David Sifri, Keith Wilson, Sarah Smolik, Michael Forte, Buddy Ullman

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.

Original language	English (US)
Pages (from-to)	101-102
Number of pages	2
Journal	BBA - Gene Structure and Expression
Volume	1217
Issue number	1
DOIs	https://doi.org/10.1016/0167-4781(94)90132-5
State	Published - Jan 18 1994

Keywords

(D. melanogaster)
Cloning
Development
Guanylate
Nucleotide sequence
Purine

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

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10.1016/0167-4781(94)90132-5

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title = "Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase",

abstract = "A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.",

keywords = "(D. melanogaster), Cloning, Development, Guanylate, Nucleotide sequence, Purine",

author = "Sifri, {C. David} and Keith Wilson and Sarah Smolik and Michael Forte and Buddy Ullman",

note = "Funding Information: This work was supportedby grantA I23682f rom the National Instituteo f Allergy and InfectiousD isease. Buddy Ullman is a BurroughsW ellcomeF und Scholar in MolecularP arasitologya,n dthis work was supported in part by a grant from The BurroughsW ellcome Fund.",

year = "1994",

month = jan,

day = "18",

doi = "10.1016/0167-4781(94)90132-5",

language = "English (US)",

volume = "1217",

pages = "101--102",

journal = "BBA - Gene Structure and Expression",

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TY - JOUR

T1 - Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase

AU - Sifri, C. David

AU - Wilson, Keith

AU - Smolik, Sarah

AU - Forte, Michael

AU - Ullman, Buddy

N1 - Funding Information: This work was supportedby grantA I23682f rom the National Instituteo f Allergy and InfectiousD isease. Buddy Ullman is a BurroughsW ellcomeF und Scholar in MolecularP arasitologya,n dthis work was supported in part by a grant from The BurroughsW ellcome Fund.

PY - 1994/1/18

Y1 - 1994/1/18

N2 - A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.

AB - A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.

KW - (D. melanogaster)

KW - Cloning

KW - Development

KW - Guanylate

KW - Nucleotide sequence

KW - Purine

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U2 - 10.1016/0167-4781(94)90132-5

DO - 10.1016/0167-4781(94)90132-5

M3 - Article

C2 - 7904480

AN - SCOPUS:27244462078

SN - 0167-4781

VL - 1217

SP - 101

EP - 102

JO - BBA - Gene Structure and Expression

JF - BBA - Gene Structure and Expression

IS - 1

ER -

Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase

Abstract

Keywords

ASJC Scopus subject areas

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