Cloning and sequence analysis of a Drosophila melanogaster cDNA encoding IMP dehydrogenase

C. David Sifri, Keith Wilson, Sarah Smolik, Michael Forte, Buddy Ullman

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

A cDNA encoding the entire Drosophila melanogaster IMP dehydrogenase (IMPDH) protein was isolated and sequenced. Translation of the impdh cDNA nucleotide sequence indicated that the Drosophila IMPDH exhibited 48% and 65% amino acid identity to the Leishmania donovani and human isoform II counterparts, respectively. Northern analysis revealed that expression of the 2.4 kb impdh transcript was equivalent in adult Drosophila head and body and that impdh expression was developmentally regulated. In situ hybridization of the cloned impdh cDNA probe to Drosophila salivary gland chromosomes indicated that the impdh gene is located at position 9E1-4 on the X chromosome.

Original languageEnglish (US)
Pages (from-to)101-102
Number of pages2
JournalBBA - Gene Structure and Expression
Volume1217
Issue number1
DOIs
StatePublished - Jan 18 1994

Keywords

  • (D. melanogaster)
  • Cloning
  • Development
  • Guanylate
  • Nucleotide sequence
  • Purine

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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