Cloning and functional expression of a rat heart KATP channel

M. L J Ashford, C. T. Bond, T. A. Blair, John Adelman

Research output: Contribution to journalArticle

166 Citations (Scopus)

Abstract

POTASSIUM channels that are ATP-sensitive (KATP) couple membrane potential to the metabolic status of the cell. KATP channels are inhibited by intracellular ATP and are stimulated by intracellular nucleotide diphosphates1. KATP channels are important regulators of secretory processes and muscle contraction, and are targets for therapeutic treatment of type II diabetes by the inhibitory sulphonylureas2 and for hypertension by activators such as pinacidil3. In cardiac tissue, KATP channels are central regulators of post-ischaemic cardioprotection4,5. Electrophysiological and pharmacological characteristics vary among KATP channels recorded from diverse tissues suggesting extensive molecular heterogeneity1. A complementary DNA encoding a KATP channel was isolated from rat heart using the polymerase chain reaction. We report here that the expressed channels possess all of the essential features of native cardiac KATP channels, including sensitivity to intracellular nucleotides. In addition the cloned channels are activated by the potassium channel opener, pinacidil, but are not inhibited by the sulphonylurea, glibenclamide.

Original languageEnglish (US)
Pages (from-to)456-459
Number of pages4
JournalNature
Volume370
Issue number6489
StatePublished - 1994

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KATP Channels
Organism Cloning
Nucleotides
Adenosine Triphosphate
Pinacidil
Glyburide
Secretory Pathway
Potassium Channels
Muscle Contraction
Membrane Potentials
Type 2 Diabetes Mellitus
Complementary DNA
Pharmacology
Hypertension
Polymerase Chain Reaction
Therapeutics

ASJC Scopus subject areas

  • General

Cite this

Ashford, M. L. J., Bond, C. T., Blair, T. A., & Adelman, J. (1994). Cloning and functional expression of a rat heart KATP channel. Nature, 370(6489), 456-459.

Cloning and functional expression of a rat heart KATP channel. / Ashford, M. L J; Bond, C. T.; Blair, T. A.; Adelman, John.

In: Nature, Vol. 370, No. 6489, 1994, p. 456-459.

Research output: Contribution to journalArticle

Ashford, MLJ, Bond, CT, Blair, TA & Adelman, J 1994, 'Cloning and functional expression of a rat heart KATP channel', Nature, vol. 370, no. 6489, pp. 456-459.
Ashford MLJ, Bond CT, Blair TA, Adelman J. Cloning and functional expression of a rat heart KATP channel. Nature. 1994;370(6489):456-459.
Ashford, M. L J ; Bond, C. T. ; Blair, T. A. ; Adelman, John. / Cloning and functional expression of a rat heart KATP channel. In: Nature. 1994 ; Vol. 370, No. 6489. pp. 456-459.
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