Cloning and expression of human and rat D1 dopamine receptors

Qun Yong Zhou, David Grandy, Lisa Thambi, Jake A. Kushner, Hubert H M Van Tol, Roger Cone, David Pribnow, John Salon, James R. Bunzow, Olivier Civelli

    Research output: Contribution to journalArticle

    486 Citations (Scopus)

    Abstract

    THE importance of the dopaminergic system in brain function has been emphasized by its association with neurological and psychiatric disorders such as Parkinson's disease and schizophrenia. On the basis of their biochemical and pharmacological characteristics, dopamine receptors are classified into D1 and D2 subtypes1,2. As the most abundant dopamine receptor in the central nervous system, D1 receptors seem to mediate some behavioural responses3, modulate activity of D2 dopamine receptors4,5, and regulate neuron growth and differentiation6. The D2 dopamine receptor has been cloned by low-stringency screening7. We report here the cloning of human and rat D1 dopamine receptors by applying an approach based on the polymerase chain reaction8. The cloned human D1 dopamine receptor has been characterized on the basis of four criteria: the deduced amino-acid sequence, which reveals that it is a G protein-coupled receptor; the tissue distribution of its messenger RNA, which is compatible with that of the D1 dopamine receptor; its pharmacological profile when transfected into COS-7 cells; and its ability to stimulate the accumulation of cyclic AMP in human 293 cells.

    Original languageEnglish (US)
    Pages (from-to)76-80
    Number of pages5
    JournalNature
    Volume347
    Issue number6288
    StatePublished - Sep 6 1990

    Fingerprint

    Dopamine Receptors
    Organism Cloning
    Pharmacology
    Dopamine D1 Receptors
    Aptitude
    Dopamine D2 Receptors
    COS Cells
    Tissue Distribution
    G-Protein-Coupled Receptors
    Nervous System Diseases
    Cyclic AMP
    Parkinson Disease
    Psychiatry
    Amino Acid Sequence
    Dopamine
    Schizophrenia
    Central Nervous System
    Neurons
    Messenger RNA
    Brain

    ASJC Scopus subject areas

    • General

    Cite this

    Zhou, Q. Y., Grandy, D., Thambi, L., Kushner, J. A., Van Tol, H. H. M., Cone, R., ... Civelli, O. (1990). Cloning and expression of human and rat D1 dopamine receptors. Nature, 347(6288), 76-80.

    Cloning and expression of human and rat D1 dopamine receptors. / Zhou, Qun Yong; Grandy, David; Thambi, Lisa; Kushner, Jake A.; Van Tol, Hubert H M; Cone, Roger; Pribnow, David; Salon, John; Bunzow, James R.; Civelli, Olivier.

    In: Nature, Vol. 347, No. 6288, 06.09.1990, p. 76-80.

    Research output: Contribution to journalArticle

    Zhou, QY, Grandy, D, Thambi, L, Kushner, JA, Van Tol, HHM, Cone, R, Pribnow, D, Salon, J, Bunzow, JR & Civelli, O 1990, 'Cloning and expression of human and rat D1 dopamine receptors', Nature, vol. 347, no. 6288, pp. 76-80.
    Zhou QY, Grandy D, Thambi L, Kushner JA, Van Tol HHM, Cone R et al. Cloning and expression of human and rat D1 dopamine receptors. Nature. 1990 Sep 6;347(6288):76-80.
    Zhou, Qun Yong ; Grandy, David ; Thambi, Lisa ; Kushner, Jake A. ; Van Tol, Hubert H M ; Cone, Roger ; Pribnow, David ; Salon, John ; Bunzow, James R. ; Civelli, Olivier. / Cloning and expression of human and rat D1 dopamine receptors. In: Nature. 1990 ; Vol. 347, No. 6288. pp. 76-80.
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