Cloning and expression of a rat somatostatin receptor enriched in brain

Xiao Jiang Li, Michael Forte, R. Alan North, Christopher A. Ross, Solomon H. Snyder

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

The tetradecapeptide somatostatin (SRIF) is a hormone release-inhibiting substance that mediates diverse effects in brain and peripheral organs via specific receptors. A cDNA encoding a rat SRIF receptor was identified by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed in rat brain. The complete cDNA encodes a protein of 391 amino acids with seven potential transmembrane domains. Expression of the cDNA product in transfected COS-7 cell lines provides the same high affinity of binding to [125I-Tyr11]SRIF-14 as that of rat cerebral cortex tissues. However, the binding of [125I-Tyr11] SRIF-14 to cloned rat SRIF receptor is not displaced by MK678, a SRIF analog that partially displaces [125I-Tyr11]SRIF-14 binding sites in membranes of rat cerebral cortex. Northern analysis and in situ hybridization indicate that mRNA (4.0 kilobases) for cloned rat SRIF receptor is preferentially expressed in rat brain regions such as cerebral cortex and hippocampus with no detectable expression in most peripheral organs. This pattern contrasts with the exclusive peripheral expression of a recently cloned human SRIF receptor. The cDNA probe of rat receptor detects mRNA from mouse brain but not from human cerebral cortex and cerebellum.

Original languageEnglish (US)
Pages (from-to)21307-21312
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number30
StatePublished - Oct 25 1992

Fingerprint

Somatostatin Receptors
Cloning
Rats
Organism Cloning
Brain
Complementary DNA
Cerebral Cortex
Messenger RNA
DNA Primers
COS Cells
Polymerase chain reaction
Somatostatin
Membrane Potentials
Cerebellum
In Situ Hybridization
Amplification
Hippocampus
Binding Sites
Cells
Hormones

ASJC Scopus subject areas

  • Biochemistry

Cite this

Li, X. J., Forte, M., North, R. A., Ross, C. A., & Snyder, S. H. (1992). Cloning and expression of a rat somatostatin receptor enriched in brain. Journal of Biological Chemistry, 267(30), 21307-21312.

Cloning and expression of a rat somatostatin receptor enriched in brain. / Li, Xiao Jiang; Forte, Michael; North, R. Alan; Ross, Christopher A.; Snyder, Solomon H.

In: Journal of Biological Chemistry, Vol. 267, No. 30, 25.10.1992, p. 21307-21312.

Research output: Contribution to journalArticle

Li, XJ, Forte, M, North, RA, Ross, CA & Snyder, SH 1992, 'Cloning and expression of a rat somatostatin receptor enriched in brain', Journal of Biological Chemistry, vol. 267, no. 30, pp. 21307-21312.
Li, Xiao Jiang ; Forte, Michael ; North, R. Alan ; Ross, Christopher A. ; Snyder, Solomon H. / Cloning and expression of a rat somatostatin receptor enriched in brain. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 30. pp. 21307-21312.
@article{de836551cdfd48b5bb7c2642bb5d08c0,
title = "Cloning and expression of a rat somatostatin receptor enriched in brain",
abstract = "The tetradecapeptide somatostatin (SRIF) is a hormone release-inhibiting substance that mediates diverse effects in brain and peripheral organs via specific receptors. A cDNA encoding a rat SRIF receptor was identified by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed in rat brain. The complete cDNA encodes a protein of 391 amino acids with seven potential transmembrane domains. Expression of the cDNA product in transfected COS-7 cell lines provides the same high affinity of binding to [125I-Tyr11]SRIF-14 as that of rat cerebral cortex tissues. However, the binding of [125I-Tyr11] SRIF-14 to cloned rat SRIF receptor is not displaced by MK678, a SRIF analog that partially displaces [125I-Tyr11]SRIF-14 binding sites in membranes of rat cerebral cortex. Northern analysis and in situ hybridization indicate that mRNA (4.0 kilobases) for cloned rat SRIF receptor is preferentially expressed in rat brain regions such as cerebral cortex and hippocampus with no detectable expression in most peripheral organs. This pattern contrasts with the exclusive peripheral expression of a recently cloned human SRIF receptor. The cDNA probe of rat receptor detects mRNA from mouse brain but not from human cerebral cortex and cerebellum.",
author = "Li, {Xiao Jiang} and Michael Forte and North, {R. Alan} and Ross, {Christopher A.} and Snyder, {Solomon H.}",
year = "1992",
month = "10",
day = "25",
language = "English (US)",
volume = "267",
pages = "21307--21312",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "30",

}

TY - JOUR

T1 - Cloning and expression of a rat somatostatin receptor enriched in brain

AU - Li, Xiao Jiang

AU - Forte, Michael

AU - North, R. Alan

AU - Ross, Christopher A.

AU - Snyder, Solomon H.

PY - 1992/10/25

Y1 - 1992/10/25

N2 - The tetradecapeptide somatostatin (SRIF) is a hormone release-inhibiting substance that mediates diverse effects in brain and peripheral organs via specific receptors. A cDNA encoding a rat SRIF receptor was identified by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed in rat brain. The complete cDNA encodes a protein of 391 amino acids with seven potential transmembrane domains. Expression of the cDNA product in transfected COS-7 cell lines provides the same high affinity of binding to [125I-Tyr11]SRIF-14 as that of rat cerebral cortex tissues. However, the binding of [125I-Tyr11] SRIF-14 to cloned rat SRIF receptor is not displaced by MK678, a SRIF analog that partially displaces [125I-Tyr11]SRIF-14 binding sites in membranes of rat cerebral cortex. Northern analysis and in situ hybridization indicate that mRNA (4.0 kilobases) for cloned rat SRIF receptor is preferentially expressed in rat brain regions such as cerebral cortex and hippocampus with no detectable expression in most peripheral organs. This pattern contrasts with the exclusive peripheral expression of a recently cloned human SRIF receptor. The cDNA probe of rat receptor detects mRNA from mouse brain but not from human cerebral cortex and cerebellum.

AB - The tetradecapeptide somatostatin (SRIF) is a hormone release-inhibiting substance that mediates diverse effects in brain and peripheral organs via specific receptors. A cDNA encoding a rat SRIF receptor was identified by use of degenerate oligonucleotide primers and polymerase chain reaction amplification of cDNA prepared from transcripts expressed in rat brain. The complete cDNA encodes a protein of 391 amino acids with seven potential transmembrane domains. Expression of the cDNA product in transfected COS-7 cell lines provides the same high affinity of binding to [125I-Tyr11]SRIF-14 as that of rat cerebral cortex tissues. However, the binding of [125I-Tyr11] SRIF-14 to cloned rat SRIF receptor is not displaced by MK678, a SRIF analog that partially displaces [125I-Tyr11]SRIF-14 binding sites in membranes of rat cerebral cortex. Northern analysis and in situ hybridization indicate that mRNA (4.0 kilobases) for cloned rat SRIF receptor is preferentially expressed in rat brain regions such as cerebral cortex and hippocampus with no detectable expression in most peripheral organs. This pattern contrasts with the exclusive peripheral expression of a recently cloned human SRIF receptor. The cDNA probe of rat receptor detects mRNA from mouse brain but not from human cerebral cortex and cerebellum.

UR - http://www.scopus.com/inward/record.url?scp=0026802434&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026802434&partnerID=8YFLogxK

M3 - Article

C2 - 1400442

AN - SCOPUS:0026802434

VL - 267

SP - 21307

EP - 21312

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 30

ER -