Cloning and expression in Escherichia coli of the gene encoding the heat-modifiable major outer membrane protein of Haemophilus influenzae type b

Medicine & Life Sciences

• Haemophilus influenzae type b 100%
• Organism Cloning 70%
• Membrane Proteins 63%
• Hot Temperature 59%
• Escherichia coli 54%
• Genes 31%
• Bacteriophages 20%
• Proteins 20%
• Escherichia coli Proteins 16%
• outer membrane protein P1, Haemophilus influenzae type b 16%
• DNA 15%
• Sodium Dodecyl Sulfate 15%
• Western Blotting 14%
• Polyacrylamide Gel Electrophoresis 13%
• Molecular Weight 11%
• Bacteriophage P1 11%
• Plasmids 11%
• Coliphages 10%
• Monoclonal Antibodies 10%
• Lac Operon 8%
• Heating 7%
• Bacteremia 7%
• Meningitis 7%
• Recombinant Proteins 6%
• Radioimmunoassay 6%
• Serum 6%
• Animal Models 5%
• Membranes 4%
• Antibodies 3%
• Growth 3%

Agriculture & Biology

• Haemophilus influenzae 95%
• outer membrane proteins 89%
• molecular cloning 58%
• Escherichia coli 49%
• heat 42%
• phages 29%
• genes 26%
• proteins 17%
• protein synthesis 14%
• DNA 13%
• monoclonal antibodies 11%
• Western blotting 10%
• polyacrylamide gel electrophoresis 10%
• meningitis 8%
• plasmid vectors 8%
• molecular weight 7%
• gene induction 7%
• recombinant proteins 6%
• radioimmunoassays 6%
• protective effect 6%
• animal models 5%
• plasmids 4%
• transcription (genetics) 4%
• screening 4%
• antibodies 4%