Cloning and developmental regulation of alpha 1 acid glycoprotein in swine.

R. T. Stone, Richard Maurer

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

A cDNA clone of porcine alpha 1 acid glycoprotein (alpha 1AGP) has been isolated and sequenced. Sequence homologies between porcine, human, and rat indicate that porcine alpha 1AGP is similar in structure to the rat and human proteins. RNA blots from days 40, 60, 80, and 110 fetal, newborn, and adult livers showed that alpha 1AGP mRNA is relatively abundant throughout fetal development, particularly at the later stages and in the newborn; there is a rapid decline in abundance following birth. From birth to 3 days of age, there is a three- to four-fold decline in abundance, and alpha 1AGP mRNA is approximately 100 times less abundant in the adult liver than in that of perinatal pigs. Southern blots showed that alpha 1AGP is probably a single-copy gene. The isolation of a cloned cDNA for porcine alpha 1AGP provides a tool to investigate the molecular mechanisms involved in the developmental regulation of the gene and to correlate changes in gene expression during development with fetal growth and well being.

Original languageEnglish (US)
Pages (from-to)295-304
Number of pages10
JournalDevelopmental Genetics
Volume8
Issue number4
StatePublished - 1987
Externally publishedYes

Fingerprint

Orosomucoid
Organism Cloning
Swine
Fetal Development
Complementary DNA
Parturition
Developmental Genes
Messenger RNA
Liver
Sequence Homology
Southern Blotting
Clone Cells
RNA
Gene Expression
Genes
Proteins

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Genetics

Cite this

Cloning and developmental regulation of alpha 1 acid glycoprotein in swine. / Stone, R. T.; Maurer, Richard.

In: Developmental Genetics, Vol. 8, No. 4, 1987, p. 295-304.

Research output: Contribution to journalArticle

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