TY - JOUR
T1 - Cloning and characterization of two catechol 1,2-dioxygenase genes from Acinetobacter radioresistens S13
AU - Caposio, Patrizia
AU - Pessione, Enrica
AU - Giuffrida, Gabriella
AU - Conti, Amedeo
AU - Landolfo, Santo
AU - Giunta, Carlo
AU - Gribaudo, Giorgio
N1 - Funding Information:
This work was supported by grants from the MURST (60%) and from Consorzio Interuniversitario per le Biotec-nologie (CIB).
PY - 2002
Y1 - 2002
N2 - Two novel catechol 1,2-dioxygenase (C 1,2-O) genes have been isolated from an Acinetobacter radioresistens strain that grows on phenol or benzoate as sole carbon and energy source. Designated as catAA and catAB, they encode proteins composed of 314 and 306 amino acids, whose deduced sequences indicate that they have approximately 53% identity, whereas their NH2-terminal and COOH-terminal regions have no sequences in common. This may explain their different thermal and pH stability. Polyclonal antibodies raised against an amino-terminal CatAA peptide or the whole CatAB protein were used to establish their inducible and differential expression patterns upon bacterial growth in phenol or benzoate. The CatAA protein (IsoA) was induced by both phenol and benzoate though with different kinetics, whereas the catAB product (IsoB) was constitutively produced at low levels that increased only during growth in the presence of benzoate.
AB - Two novel catechol 1,2-dioxygenase (C 1,2-O) genes have been isolated from an Acinetobacter radioresistens strain that grows on phenol or benzoate as sole carbon and energy source. Designated as catAA and catAB, they encode proteins composed of 314 and 306 amino acids, whose deduced sequences indicate that they have approximately 53% identity, whereas their NH2-terminal and COOH-terminal regions have no sequences in common. This may explain their different thermal and pH stability. Polyclonal antibodies raised against an amino-terminal CatAA peptide or the whole CatAB protein were used to establish their inducible and differential expression patterns upon bacterial growth in phenol or benzoate. The CatAA protein (IsoA) was induced by both phenol and benzoate though with different kinetics, whereas the catAB product (IsoB) was constitutively produced at low levels that increased only during growth in the presence of benzoate.
KW - Acinetobacter radioresistens S13
KW - Catechol 1,2-dioxygenase
KW - Expression patterns
UR - http://www.scopus.com/inward/record.url?scp=0036175003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0036175003&partnerID=8YFLogxK
U2 - 10.1016/S0923-2508(01)01290-6
DO - 10.1016/S0923-2508(01)01290-6
M3 - Article
C2 - 11900265
AN - SCOPUS:0036175003
SN - 0923-2508
VL - 153
SP - 69
EP - 74
JO - Research in Microbiology
JF - Research in Microbiology
IS - 2
ER -