Cloning and characterization of MVP17: A developmentally regulated myelin protein in oligodendrocytes

T. Kim, K. Fiedler, Dana Madison, W. H. Krueger, S. E. Pfeiffer

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

The remarkable quantities of myelin membrane produced by oligodendrocytes has led us to examine the mechanisms involved in the sorting and transport of proteins and lipids during myelinogenesis. Noting that it has been proposed that proteins destined for the apical surface of polarized epithelial cells co-cluster with glycolipid-rich microdomains during sorting and transport from the trans-Golgi network (Simons and van Meer: Biochemistry 27:6197- 6202, 1988; Simons and Wandinger-Ness: Cell 62:207-210, 1990), we hypothesized that the glycolipid-rich oligodendrocytes may adopt this mechanism for myelinogenesis. Protein-lipid complexes from oligodendrocytes and myelin were isolated utilizing detergent insolubility and two-dimensional gel electrophoresis. A developmentally regulated protein, MVP17 (myelin vesicular protein of 17 kDa), was identified. Microsequencing of the N- terminal peptide revealed a high homology to human T-cell MAL protein (Alonso and Weissman: Proc Natl Acad Sci USA 84:1997-2001, 1987). The corresponding MVP17 cDNA was isolated from an oligodendrocyte cDNA library. The predicted protein sequence showed 88.9% identity with MAL, and the hydrophobicity profile suggested four transmembrane domains. In vitro translation demonstrated a signal at the deduced Mr of ~17 kDa. Northern analyses indicated that MVP17 mRNA expression is restricted to brain and kidney and that this expression is up-regulated in oligodendrocytes and brain during the period of active myelination. These data suggest that MVP17 is involved in myelin biogenesis and/or myelin function.

Original languageEnglish (US)
Pages (from-to)413-422
Number of pages10
JournalJournal of Neuroscience Research
Volume42
Issue number3
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

Myelin Proteins
Oligodendroglia
Organism Cloning
Myelin Sheath
Glycolipids
Myelin and Lymphocyte-Associated Proteolipid Proteins
Proteins
trans-Golgi Network
Lipids
Electrophoresis, Gel, Two-Dimensional
Brain
Hydrophobic and Hydrophilic Interactions
Gene Library
Detergents
Biochemistry
Carrier Proteins
Complementary DNA
Epithelial Cells
Kidney
Messenger RNA

Keywords

  • detergent-insoluble complexes
  • MAL
  • myelin
  • myelinogenesis
  • protein sorting

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cloning and characterization of MVP17 : A developmentally regulated myelin protein in oligodendrocytes. / Kim, T.; Fiedler, K.; Madison, Dana; Krueger, W. H.; Pfeiffer, S. E.

In: Journal of Neuroscience Research, Vol. 42, No. 3, 1995, p. 413-422.

Research output: Contribution to journalArticle

Kim, T. ; Fiedler, K. ; Madison, Dana ; Krueger, W. H. ; Pfeiffer, S. E. / Cloning and characterization of MVP17 : A developmentally regulated myelin protein in oligodendrocytes. In: Journal of Neuroscience Research. 1995 ; Vol. 42, No. 3. pp. 413-422.
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