Cloning and Characterization of EagI YACs from Human Chromosome 21

Jeffrey C. Gingrich, Stephen R. Lowry, Wen Lin Kuo, Joe Gray, Cassandra L. Smith, Charles R. Cantori

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Yeast artificial chromosomes (YACs) were made from a total EagI digest of DNA from a mouse-human chromosome 21 hybrid cell line. Approximately 3750 YACs, corresponding to 75-125 human YACs, with an average size of approximately 100 kb were recovered. Southern hybridization indicates that the chimera frequency in this library may be less than 3%. Thirty-four of the human Eag I YACs were regionally assigned by a number of methods. Some YACs were regionally assigned to one of six chromosome regions by hybridization of Alu -PCR products from the YAC against Alu-PCR-amplified DNA from a panel of hybrid cell lines that contain various parts of chromosome 21. Additional YACs were regionally assigned by fluorescence in situ hybridization using either biotinylated Alu-PCR products or yeast genomic DNA from the YAC-containing strains as probes. The regionally assigned Eag I YACs are located preferentially in two regions of the chromosome: near the q telomere and in the p-arm ribosomal gene region.

Original languageEnglish (US)
Pages (from-to)228-230
Number of pages3
JournalGenomics
Volume15
Issue number1
DOIs
StatePublished - Jan 1993
Externally publishedYes

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Yeast Artificial Chromosomes
Chromosomes, Human, Pair 21
Human Chromosomes
Organism Cloning
Hybrid Cells
Polymerase Chain Reaction
DNA
Human Artificial Chromosomes
Chromosomes
Cell Line
Telomere
Fluorescence In Situ Hybridization
Libraries
Yeasts

ASJC Scopus subject areas

  • Genetics

Cite this

Gingrich, J. C., Lowry, S. R., Kuo, W. L., Gray, J., Smith, C. L., & Cantori, C. R. (1993). Cloning and Characterization of EagI YACs from Human Chromosome 21. Genomics, 15(1), 228-230. https://doi.org/10.1006/geno.1993.1043

Cloning and Characterization of EagI YACs from Human Chromosome 21. / Gingrich, Jeffrey C.; Lowry, Stephen R.; Kuo, Wen Lin; Gray, Joe; Smith, Cassandra L.; Cantori, Charles R.

In: Genomics, Vol. 15, No. 1, 01.1993, p. 228-230.

Research output: Contribution to journalArticle

Gingrich, JC, Lowry, SR, Kuo, WL, Gray, J, Smith, CL & Cantori, CR 1993, 'Cloning and Characterization of EagI YACs from Human Chromosome 21', Genomics, vol. 15, no. 1, pp. 228-230. https://doi.org/10.1006/geno.1993.1043
Gingrich JC, Lowry SR, Kuo WL, Gray J, Smith CL, Cantori CR. Cloning and Characterization of EagI YACs from Human Chromosome 21. Genomics. 1993 Jan;15(1):228-230. https://doi.org/10.1006/geno.1993.1043
Gingrich, Jeffrey C. ; Lowry, Stephen R. ; Kuo, Wen Lin ; Gray, Joe ; Smith, Cassandra L. ; Cantori, Charles R. / Cloning and Characterization of EagI YACs from Human Chromosome 21. In: Genomics. 1993 ; Vol. 15, No. 1. pp. 228-230.
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