Cloning and Characterization of EagI YACs from Human Chromosome 21

Jeffrey C. Gingrich, Stephen R. Lowry, Wen Lin Kuo, Joe Gray, Cassandra L. Smith, Charles R. Cantori

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Yeast artificial chromosomes (YACs) were made from a total EagI digest of DNA from a mouse-human chromosome 21 hybrid cell line. Approximately 3750 YACs, corresponding to 75-125 human YACs, with an average size of approximately 100 kb were recovered. Southern hybridization indicates that the chimera frequency in this library may be less than 3%. Thirty-four of the human Eag I YACs were regionally assigned by a number of methods. Some YACs were regionally assigned to one of six chromosome regions by hybridization of Alu -PCR products from the YAC against Alu-PCR-amplified DNA from a panel of hybrid cell lines that contain various parts of chromosome 21. Additional YACs were regionally assigned by fluorescence in situ hybridization using either biotinylated Alu-PCR products or yeast genomic DNA from the YAC-containing strains as probes. The regionally assigned Eag I YACs are located preferentially in two regions of the chromosome: near the q telomere and in the p-arm ribosomal gene region.

Original languageEnglish (US)
Pages (from-to)228-230
Number of pages3
JournalGenomics
Volume15
Issue number1
DOIs
StatePublished - Jan 1993

ASJC Scopus subject areas

  • Genetics

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    Gingrich, J. C., Lowry, S. R., Kuo, W. L., Gray, J., Smith, C. L., & Cantori, C. R. (1993). Cloning and Characterization of EagI YACs from Human Chromosome 21. Genomics, 15(1), 228-230. https://doi.org/10.1006/geno.1993.1043