Cloning and characterization of cDNAs encoding human gastrin-releasing peptide

E. R. Spindel, W. W. Chin, J. Price, L. H. Rees, G. M. Besser, J. F. Habener

    Research output: Contribution to journalArticle

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    Abstract

    We have prepared and cloned cDNAs derived from poly(A)+ RNA from a human pulmonary carcinoid tumor rich in immunoreactivity to gastrin-releasing peptide, a peptide closely related in structure to amphibian bombesin. Mixtures of synthetic oligodeoxyribonucleotides corresponding to amphibian bombesin were used as hybridization probes to screen a cDNA library prepared from the tumor RNA. Sequencing of the recombinant plasmids shows that human gastrin-releasing peptide (hGRP) mRNA encodes a precursor of 148 amino acids containing a typical signal sequence, hGRP consisting of 27 or 28 amino acids, and a carboxyl-terminal extension peptide. hGRP is flanked at its carboxyl terminus by two basic amino acids, following a glycine used for amidation of the carboxyl-terminal methionine. RNA blot analyses of tumor RNA show a major mRNA of 900 bases and a minor mRNA of 850 bases. Blot hybridization analyses using human genomic DNA are consistent with a single hGRP-encoding gene. The presence of two mRNAs encoding the hGRP precursor protein in the face of a single hGRP gene raises the possibility of alternative processing of the single RNA transcript.

    Original languageEnglish (US)
    Pages (from-to)5699-5703
    Number of pages5
    JournalProceedings of the National Academy of Sciences of the United States of America
    Volume81
    Issue number18 I
    DOIs
    StatePublished - Jan 1 1984

    ASJC Scopus subject areas

    • General

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