Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses

C. S. Tailor, A. Nouri, C. G. Lee, C. Kozak, David Kabat

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Abstract

Xenotropic and polytropic murine leukemia viruses (X-MLVs and P-MLVs) cross-interfere to various extents in non-mouse species and in wild Asian mice, suggesting that they might use a common receptor for infection. Consistent with this hypothesis, the susceptibility of some wild mice to X- MLVs has been mapped to the P-MLV receptor locus at the distal end of mouse chromosome 1. In this study, we report the isolation and characterization of a cDNA for the human X-MLV cell surface receptor (X-receptor) by using a human T lymphocyte cDNA library in a retroviral vector. The predicted X- receptor contains 696 amino acids with multiple hydrophobic potential membrane-spanning sequences and with weak homologies to the yeast proteins SYG1, of unknown function, and PHO81, which has been implicated in a system that regulates transport of inorganic phosphate. Expression of the X-receptor in Chinese hamster ovary cells, which are substantially resistant to P-MLVs and to X-MLVs, made them susceptible to both of these virus groups. The mouse homologue of the X-receptor was mapped by hybridization to the distal end of chromosome 1 at the same position as the P-MLV receptor gene Rmc1. These results strongly support the hypothesis that a common gene encodes the receptors for X-MLVs and P-MLVs, with the human X-receptor preferentially mediating X-MLV infections and the homologous protein of inbred mice mediating only P-MLV infections. We propose that X-MLVs and P-MLVs comprise a single family of retroviruses that have coevolved in response to diversification in X-receptor genes of the host.

Original languageEnglish (US)
Pages (from-to)927-932
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number3
DOIs
StatePublished - Feb 2 1999

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Murine Leukemia Viruses
Cell Surface Receptors
Organism Cloning
Chromosomes, Human, Pair 1
Infection
Genes
Fungal Proteins
Retroviridae
Cricetulus
Gene Library
Membrane Potentials
Ovary
Complementary DNA
Phosphates
Viruses
T-Lymphocytes
Amino Acids
Proteins

ASJC Scopus subject areas

  • Genetics
  • General

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Cloning and characterization of a cell surface receptor for xenotropic and polytropic murine leukemia viruses. / Tailor, C. S.; Nouri, A.; Lee, C. G.; Kozak, C.; Kabat, David.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 3, 02.02.1999, p. 927-932.

Research output: Contribution to journalArticle

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