Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase

Manuel Esguerra, Jing Wang, Christine D. Foster, John Adelman, R. Alan North, Irwin B. Levitan

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

CALCIUM-DEPENDENT potassium (KCa) channels carry ionic currents that regulate important cellular functions1-3. Like some other ion channels4-10, KCa channels are modulated by protein phosphorylation2,11,15-21. The recent cloning of complementary DNAs encoding Slo KCa, channels12-14 has enabled KCa channel modulation to be investigated. We report here that protein phosphorylation modulates the activity of Drosophila Slo KCa channels expressed in Xenopus oocytes. Application of ATP-γS to detached membrane patches increases Slo channel activity by shifting channel voltage sensitivity. This modulation is blocked by a specific inhibitor of cyclic AMP-dependent protein kinase (PKA). Mutation of a single serine residue in the channel protein also blocks modulation by ATP-γS, demonstrating that phosphorylation of the Slo channel protein itself modulates channel activity. The results also indicate that KCa channels in oocyte membrane patches can be modulated by an endogenous PKA-like protein kinase which remains functionally associated with the channels in the detached patch.

Original languageEnglish (US)
Pages (from-to)563-565
Number of pages3
JournalNature
Volume369
Issue number6481
StatePublished - Jun 16 1994

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Protein Kinases
Oocytes
Proteins
Adenosine Triphosphate
Phosphorylation
Membranes
Potassium Channels
Xenopus
Serine
Drosophila
Organism Cloning
Complementary DNA
Ions
Mutation

ASJC Scopus subject areas

  • General

Cite this

Esguerra, M., Wang, J., Foster, C. D., Adelman, J., North, R. A., & Levitan, I. B. (1994). Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase. Nature, 369(6481), 563-565.

Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase. / Esguerra, Manuel; Wang, Jing; Foster, Christine D.; Adelman, John; North, R. Alan; Levitan, Irwin B.

In: Nature, Vol. 369, No. 6481, 16.06.1994, p. 563-565.

Research output: Contribution to journalArticle

Esguerra, M, Wang, J, Foster, CD, Adelman, J, North, RA & Levitan, IB 1994, 'Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase', Nature, vol. 369, no. 6481, pp. 563-565.
Esguerra M, Wang J, Foster CD, Adelman J, North RA, Levitan IB. Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase. Nature. 1994 Jun 16;369(6481):563-565.
Esguerra, Manuel ; Wang, Jing ; Foster, Christine D. ; Adelman, John ; North, R. Alan ; Levitan, Irwin B. / Cloned Ca2+-dependent K+ channel modulated by a functionally associated protein kinase. In: Nature. 1994 ; Vol. 369, No. 6481. pp. 563-565.
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