Clonal Interactions in a Human Squamous Cell Carcinoma

James E. Spellman, Molly Kulesz-Martin, Leslie E. Blumenson, Michael A. Beckett, Ralph R. Weichselbaum

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

F.2a and B.2, cell clones of the human squamous cell carcinoma SCC-12, were examined to characterize their interactions through the expression of growth factors. F.2a was nontumorigenic yet B.2 was fully tumorigenic when injected into the flanks of athymic nude mice. Combination injections of F.2a and a subtumorigenic level of B.2 produced tumors. F.2a and B.2 overexpressed the 4.8-kb transcript for transforming growth factor-α (TGF-α) as well as the 10.5- and 5.8kb transcripts for the epidermal growth factor receptor. Neither clone expressed the transcript for epidermal growth factor, while both expressed transcripts for insulin-like growth factor-I (IGF-I) of 8.15, 4.9, and 1.6 kb and transcripts for its receptor of 8.5 and 6.5 kb. Conditioned medium (CM) from either clone stimulated the growth of itself and the other clone in tissue culture. Both clones produced intracellular TGF-α detectable by immunohistochemical staining, but not detectable in CM by enzyme-linked immunosorption assay. IGF-I was detected at low levels in CM by radioimmunoassay. Neutralizing antibodies to TGF-α but not IGF-I partially inhibit the growth of both clones in tissue culture. These results suggest that (1) TGF-α is active in autocrine signaling, (2) IGF-I is not directly stimulatory, and (3) additional factors, as yet unidentified, are present in CM and enhance tumor growth. It is concluded that human squamous cell carcinoma SCC-12 is composed of tumorigenic and nontumorigenic clones which interact to enhance growth.

Original languageEnglish (US)
Pages (from-to)165-174
Number of pages10
JournalJournal of Surgical Research
Volume58
Issue number2
DOIs
StatePublished - Feb 1995
Externally publishedYes

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Squamous Cell Carcinoma
Clone Cells
Transforming Growth Factors
Conditioned Culture Medium
Insulin-Like Growth Factor I
Growth
Nude Mice
Autocrine Communication
Neutralizing Antibodies
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Radioimmunoassay
Neoplasms
Intercellular Signaling Peptides and Proteins
Staining and Labeling
Injections
Enzymes

ASJC Scopus subject areas

  • Surgery

Cite this

Spellman, J. E., Kulesz-Martin, M., Blumenson, L. E., Beckett, M. A., & Weichselbaum, R. R. (1995). Clonal Interactions in a Human Squamous Cell Carcinoma. Journal of Surgical Research, 58(2), 165-174. https://doi.org/10.1006/jsre.1995.1026

Clonal Interactions in a Human Squamous Cell Carcinoma. / Spellman, James E.; Kulesz-Martin, Molly; Blumenson, Leslie E.; Beckett, Michael A.; Weichselbaum, Ralph R.

In: Journal of Surgical Research, Vol. 58, No. 2, 02.1995, p. 165-174.

Research output: Contribution to journalArticle

Spellman, JE, Kulesz-Martin, M, Blumenson, LE, Beckett, MA & Weichselbaum, RR 1995, 'Clonal Interactions in a Human Squamous Cell Carcinoma', Journal of Surgical Research, vol. 58, no. 2, pp. 165-174. https://doi.org/10.1006/jsre.1995.1026
Spellman, James E. ; Kulesz-Martin, Molly ; Blumenson, Leslie E. ; Beckett, Michael A. ; Weichselbaum, Ralph R. / Clonal Interactions in a Human Squamous Cell Carcinoma. In: Journal of Surgical Research. 1995 ; Vol. 58, No. 2. pp. 165-174.
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