Clonal diversity, somatic mutation, and immune memory to phosphocholine-keyhole limpet hemocyanin

M. P. Stenzel-Poore, M. B. Rittenberg

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Group II antibodies to phosphocholine (PC)-keyhole limpet hemocyanin in BALB/c mice are genetically diverse and of a defined binding phenotype which recognizes the hapten, phenyl-PC, and PC coupled to protein but not free PC. We sequenced the V regions of 14 κ and λ-bearing group II antibodies. Both types show extensive somatic mutations. The pattern of the mutations differs between κ and λ antibodies. The nature of the somatic mutation in λ chains suggests strong Ag selection on the L chain but not the H chain of the λ-bearing antibodies. The reverse pattern of selection was observed among κ-containing antibodies wherein the accumulation of replacement mutations in the CDR of the H chain appears to result from selection while changes in the L chain appear unselected. From these findings it appears that somatic mutation plays a major role in anti-PC-keyhole limpet hemocyanin memory development because all 14 antibodies displayed changes from germ-line sequences.

Original languageEnglish (US)
Pages (from-to)4123-4133
Number of pages11
JournalJournal of Immunology
Volume143
Issue number12
StatePublished - Dec 1 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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