Clinically relevant concentrations of bupivacaine inhibit rat aortic baroreceptors

K. S K Chang, M. Yang, Michael Andresen

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11 Citations (Scopus)

Abstract

Bupivacaine is clinically associated with cardiovascular toxicity. To examine the possible role of drug actions at arterial baroreceptors, we studied discharge properties of baroreceptors in an in vitro aortic nerve- aortic arch preparation from rats. We measured single fiber discharge, pressure, and aortic diameter simultaneously during perfusion of the aortic arch with bupivacaine. Perfusion mean arterial pressure was held at 80 mm Hg. Only regularly discharging, presumably myelinated, baroreceptors were studied. To assess pressure threshold, threshold frequency, and maximum discharge rate, nerve activity was evoked by slow ramps of increasing pressure (1-adrenoceptor effects, respectively. Bupivacaine was then added to the perfusate in increasing concentrations from 0.1 to 50 μM for 15 min to construct a full concentration-response curve at each level. Individual baroreceptors showed substantial depression of maximum discharge frequency and/or increases in pressure threshold at 1-5 μM bupivacaine. In overall population averages (n = 7), 5-10 μM bupivacaine clearly reduced maximum discharge and shifted the pressure threshold to higher values (P <0.01). The net result was a general depression of discharge. Concentrations as low as 10 μM bupivacaine completely blocked discharge in some baroreceptors. Inasmuch as the pressure- diameter relations were not changed, discharge relations plotted against diameter showed equivalent changes. Bupivacaine-free solution reversed the block in all cases. Our results suggest that arterial baroreceptors are substantially-to-completely blocked in the range of bupivacaine concentrations encountered during clinical crises and thus this action may contribute to the early cardiovascular sequelae observed.

Original languageEnglish (US)
Pages (from-to)501-506
Number of pages6
JournalAnesthesia and Analgesia
Volume78
Issue number3
StatePublished - 1994

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Pressoreceptors
Bupivacaine
Pressure
Thoracic Aorta
Arterial Pressure
Perfusion
Architectural Accessibility
Adrenergic Receptors

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

Cite this

Clinically relevant concentrations of bupivacaine inhibit rat aortic baroreceptors. / Chang, K. S K; Yang, M.; Andresen, Michael.

In: Anesthesia and Analgesia, Vol. 78, No. 3, 1994, p. 501-506.

Research output: Contribution to journalArticle

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