Clinical genetic testing for familial melanoma in Italy: A cooperative study

William Bruno, Paola Ghiorzo, Linda Battistuzzi, Paolo A. Ascierto, Monica Barile, Sara Gargiulo, Francesca Gensini, Sara Gliori, Michele Guida, Maurizio Lombardo, Siranoush Manoukian, Chiara Menin, Sabina Nasti, Paola Origone, Barbara Pasini, Lorenza Pastorino, Bernard Peissel, Maria Antonietta Pizzichetta, Paola Queirolo, Monica RodolfoAntonella Romanini, Maria Chiara Scaini, Alessandro Testori, Maria Grazia Tibiletti, Daniela Turchetti, Sancy A. Leachman, Giovanna Bianchi Scarrà

Research output: Contribution to journalArticlepeer-review

40 Scopus citations


Background: The Italian Society of Human Genetics' (SIGU) recommendations on genetic counseling and testing for hereditary melanoma state that clinical genetic testing can be offered to Italian melanoma families with at least two affected members. Objective: In the framework of a cooperative study, we sought to establish the frequency of cyclin-dependent kinase inhibitor 2A mutations in melanoma families that underwent clinical genetic counseling and testing in accordance with the SIGU recommendations at 9 centers in different Italian regions. Methods: Cyclin-dependent kinase inhibitor 2A testing was conducted by direct sequencing and multiplex ligation-dependent probe amplification analysis in melanoma families with at least two affected members. Results: A total of 33% (68/204) of the families harbored cyclin-dependent kinase inhibitor 2A mutations. In the 145 families with two affected members the mutation frequency was 25%. Three novel mutations, L94P, A86T, and c.407dupG, were identified among the cases and not in 200 controls. Limitations: We were unable to perform separate analyses for individual centers, as in some cases the number of families was too small. Conclusions: The availability of clinical genetic testing for melanoma to families with just two affected members in the same branch is justified in Italy in terms of the likelihood of identifying a mutation.

Original languageEnglish (US)
Pages (from-to)775-782
Number of pages8
JournalJournal of the American Academy of Dermatology
Issue number5
StatePublished - Nov 2009
Externally publishedYes


  • cyclin-dependent kinase 4
  • cyclin-dependent kinase inhibitor 2A
  • familial melanoma
  • genetic testing

ASJC Scopus subject areas

  • Dermatology


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