Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila

Kathryn Parker, Julia Maxson, Alissa Mooney, Emily A. Wiley

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Class I histone deacetylases (HDACs) regulate DNA-templated processes such as transcription. They act both at specific loci and more generally across global chromatin, contributing to acetylation patterns that may underlie large-scale chromatin dynamics. Although hypoacetylation is correlated with highly condensed chromatin, little is known about the contribution of individual HDACs to chromatin condensation mechanisms. Using the ciliated protozoan Tetrahymena thermophila, we investigated the role of a specific class I HDAC Thd1p, in the reversible condensation of global chromatin. In this system, the normal physiological response to cell starvation includes the widespread condensation of the macronuclear chromatin and general repression of gene transcription. We show that the chromatin in Thd1p-deficient cells failed to condense during starvation. The condensation failure correlated with aberrant hyperphosphorylation of histone H1 and the overexpression of CDC2, encoding the major histone H1 kinase. Changes in the rate of acetate turnover on core histones and in the distribution of acetylated lysines 9 and 23/27 on histone H3 isoforms that were found to correlate with normal chromatin condensation were absent from Thd1p mutant cells. These results point to a role for a class I HDAC in the formation of reversible higher-order chromatin structures and global genome compaction through mechanisms involving the regulation of H1 phosphorylation and core histone acetylation/deacetylation kinetics.

Original languageEnglish (US)
Pages (from-to)1913-1924
Number of pages12
JournalEukaryotic Cell
Volume6
Issue number10
DOIs
StatePublished - Oct 1 2007
Externally publishedYes

Fingerprint

Tetrahymena thermophila
histone deacetylase
Histone Deacetylases
histones
Chromatin
chromatin
Histones
acetylation
Acetylation
Starvation
starvation
transcription (genetics)
cells
physiological response
Lysine
Protozoa
phosphorylation
lysine
Protein Isoforms
phosphotransferases (kinases)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. / Parker, Kathryn; Maxson, Julia; Mooney, Alissa; Wiley, Emily A.

In: Eukaryotic Cell, Vol. 6, No. 10, 01.10.2007, p. 1913-1924.

Research output: Contribution to journalArticle

Parker, Kathryn ; Maxson, Julia ; Mooney, Alissa ; Wiley, Emily A. / Class I histone deacetylase Thd1p promotes global chromatin condensation in Tetrahymena thermophila. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 10. pp. 1913-1924.
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