Cisplatin DNA cross-links do not inhibit S-phase and cause only a G2/M arrest in Saccharomyces cerevisiae

Kenneth F. Grossmann, Jessica C. Brown, Robb E. Moses

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Cisplatin (CDDP) has been used as a DNA cross-linking agent to evaluate whether there is a specific cell cycle checkpoint response to such damage in Saccharomyces cerevisiae (S. cerevisiae). Fluorescent-activated cell sorting (FACS) analysis showed only a G2/M checkpoint, normal exit from G1 and progression through S-phase following α-factor arrest and CDDP treatment. Of the checkpoint mutants tested, rad9, rad17 and rad24, did not show increased sensitivity to CDDP compared to isogenic wild-type cells. However, other checkpoint mutants tested (mec1, mec3 and rad53) showed increased sensitivity to CDDP, as did controls with a defect in excision repair (rad1 and rad14) or a defect in recombination (rad51 and rad52). Thus, by survival and cell cycle kinetics, it appears that DNA cross-links do not inhibit entry into S-phase or slow DNA replication and that replication continues after cisplatin treatment in yeast. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)29-39
Number of pages11
JournalMutation Research - DNA Repair
Volume434
Issue number1
DOIs
StatePublished - May 14 1999

Keywords

  • Cell cycle
  • Cisplatin
  • Cross-link
  • DNA repair
  • S. cerevisiae

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Genetics

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