Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma

Tara E. Henn; Ashley N. Anderson; Yvette R. Hollett; Thomas L. Sutton; Brett S. Walker; John R. Swain; David A. Sauer; Daniel R. Clayburgh; Melissa H. Wong

doi:10.1002/hed.26692

Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma

Tara E. Henn, Ashley N. Anderson, Yvette R. Hollett, Thomas L. Sutton, Brett S. Walker, John R. Swain, David A. Sauer, Daniel R. Clayburgh, Melissa H. Wong

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Background: Levels of circulating hybrid cells (CHCs), a newly identified circulating tumor cell (CTC), correlate with disease stage and progression in cancer. We investigated their utility to risk-stratify patients with clinically N0 (cN0) oral cavity squamous cell carcinoma (OCSCC), and to identify patients with occult cervical lymph node metastases (pN+). Methods: We analyzed peripheral blood samples for CHCs with co-expression of cytokeratin (tumor) and CD45 (leukocyte) from 22 patients with cN0 OCSCC using immunofluorescence microscopy, then correlated levels with pathologic lymph node status. Results: CHC levels exceeded CTCs and correlated with the presence of both clinically overt (p = 0.002) and occult nodal metastases (p = 0.006). Conclusions: For evaluated cN0 OCSCC patients, those with cN0 → pN+ status harbored elevated CHC levels compared to patients without occult disease. Our findings highlight a promising blood-based biologic assay with potential utility to determine the necessity of surgical neck dissection for staging and treatment.

Original language	English (US)
Pages (from-to)	2193-2201
Number of pages	9
Journal	Head and Neck
Volume	43
Issue number	7
DOIs	https://doi.org/10.1002/hed.26692
State	Published - Jul 2021

Keywords

blood-based analyte
head and neck cancer
occult metastases
oral pharyngeal squamous cell carcinoma
prognostic analyte

ASJC Scopus subject areas

Otorhinolaryngology

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10.1002/hed.26692

Cite this

@article{f373f719c2f94e368fed0808a43dbe26,

title = "Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma",

abstract = "Background: Levels of circulating hybrid cells (CHCs), a newly identified circulating tumor cell (CTC), correlate with disease stage and progression in cancer. We investigated their utility to risk-stratify patients with clinically N0 (cN0) oral cavity squamous cell carcinoma (OCSCC), and to identify patients with occult cervical lymph node metastases (pN+). Methods: We analyzed peripheral blood samples for CHCs with co-expression of cytokeratin (tumor) and CD45 (leukocyte) from 22 patients with cN0 OCSCC using immunofluorescence microscopy, then correlated levels with pathologic lymph node status. Results: CHC levels exceeded CTCs and correlated with the presence of both clinically overt (p = 0.002) and occult nodal metastases (p = 0.006). Conclusions: For evaluated cN0 OCSCC patients, those with cN0 → pN+ status harbored elevated CHC levels compared to patients without occult disease. Our findings highlight a promising blood-based biologic assay with potential utility to determine the necessity of surgical neck dissection for staging and treatment.",

keywords = "blood-based analyte, head and neck cancer, occult metastases, oral pharyngeal squamous cell carcinoma, prognostic analyte",

author = "Henn, {Tara E.} and Anderson, {Ashley N.} and Hollett, {Yvette R.} and Sutton, {Thomas L.} and Walker, {Brett S.} and Swain, {John R.} and Sauer, {David A.} and Clayburgh, {Daniel R.} and Wong, {Melissa H.}",

note = "Funding Information: The authors thank Catherine Beach, Michael Parappilly, Ariana Sattler, and Yuki Chin for assistance in sample collection and processing. We acknowledge the OHSU Advanced Light Microscopy Core for technical assistance. Instrumentation used in this work was supported by grant number S10‐RR023432 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) to the Advanced Light Microscopy Core at the OHSU Jungers Center. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under award numbers U54CA112970 and P30 CA069533. Publisher Copyright: {\textcopyright} 2021 Wiley Periodicals LLC.",

year = "2021",

month = jul,

doi = "10.1002/hed.26692",

language = "English (US)",

volume = "43",

pages = "2193--2201",

journal = "Head and Neck",

issn = "1043-3074",

publisher = "Wiley-Liss Inc.",

number = "7",

}

TY - JOUR

T1 - Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma

AU - Henn, Tara E.

AU - Anderson, Ashley N.

AU - Hollett, Yvette R.

AU - Sutton, Thomas L.

AU - Walker, Brett S.

AU - Swain, John R.

AU - Sauer, David A.

AU - Clayburgh, Daniel R.

AU - Wong, Melissa H.

N1 - Funding Information: The authors thank Catherine Beach, Michael Parappilly, Ariana Sattler, and Yuki Chin for assistance in sample collection and processing. We acknowledge the OHSU Advanced Light Microscopy Core for technical assistance. Instrumentation used in this work was supported by grant number S10‐RR023432 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH) to the Advanced Light Microscopy Core at the OHSU Jungers Center. Research reported in this publication was supported by the National Cancer Institute of the National Institutes of Health under award numbers U54CA112970 and P30 CA069533. Publisher Copyright: © 2021 Wiley Periodicals LLC.

PY - 2021/7

Y1 - 2021/7

N2 - Background: Levels of circulating hybrid cells (CHCs), a newly identified circulating tumor cell (CTC), correlate with disease stage and progression in cancer. We investigated their utility to risk-stratify patients with clinically N0 (cN0) oral cavity squamous cell carcinoma (OCSCC), and to identify patients with occult cervical lymph node metastases (pN+). Methods: We analyzed peripheral blood samples for CHCs with co-expression of cytokeratin (tumor) and CD45 (leukocyte) from 22 patients with cN0 OCSCC using immunofluorescence microscopy, then correlated levels with pathologic lymph node status. Results: CHC levels exceeded CTCs and correlated with the presence of both clinically overt (p = 0.002) and occult nodal metastases (p = 0.006). Conclusions: For evaluated cN0 OCSCC patients, those with cN0 → pN+ status harbored elevated CHC levels compared to patients without occult disease. Our findings highlight a promising blood-based biologic assay with potential utility to determine the necessity of surgical neck dissection for staging and treatment.

AB - Background: Levels of circulating hybrid cells (CHCs), a newly identified circulating tumor cell (CTC), correlate with disease stage and progression in cancer. We investigated their utility to risk-stratify patients with clinically N0 (cN0) oral cavity squamous cell carcinoma (OCSCC), and to identify patients with occult cervical lymph node metastases (pN+). Methods: We analyzed peripheral blood samples for CHCs with co-expression of cytokeratin (tumor) and CD45 (leukocyte) from 22 patients with cN0 OCSCC using immunofluorescence microscopy, then correlated levels with pathologic lymph node status. Results: CHC levels exceeded CTCs and correlated with the presence of both clinically overt (p = 0.002) and occult nodal metastases (p = 0.006). Conclusions: For evaluated cN0 OCSCC patients, those with cN0 → pN+ status harbored elevated CHC levels compared to patients without occult disease. Our findings highlight a promising blood-based biologic assay with potential utility to determine the necessity of surgical neck dissection for staging and treatment.

KW - blood-based analyte

KW - head and neck cancer

KW - occult metastases

KW - oral pharyngeal squamous cell carcinoma

KW - prognostic analyte

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DO - 10.1002/hed.26692

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AN - SCOPUS:85104087672

SN - 1043-3074

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EP - 2201

JO - Head and Neck

JF - Head and Neck

IS - 7

ER -

Circulating hybrid cells predict presence of occult nodal metastases in oral cavity carcinoma

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