TY - JOUR
T1 - Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons
AU - Ikeda, Masayuki
AU - Sugiyama, Takashi
AU - Wallace, Christopher S.
AU - Gompf, Heinrich S.
AU - Yoshioka, Tohru
AU - Miyawaki, Atsushi
AU - Allen, Charles N.
N1 - Funding Information:
We thank Drs. Gary Banker (Oregon Health & Science University) and Sato Honma (Hokkaido University) for their advice on neuronal cell cultures and MEAD recordings. The neuron-specific enolase promoter was a gift from Dr. Kenji Sakimura (Nigata University). This work is supported in part by a grant from the NIH (NS036607) to C.N.A., a grant for Research for the Future Program (96L00310) from Japan Society for the Promotion of Science to T.Y., a grant-in-aid for scientific research (B14380372) from the Ministry of Education, Culture, Sports, Science and Technology Japan to M.I., and a grant from Ono Pharmaceutical Company to C.N.A, M.I., and T.Y.
PY - 2003/4/24
Y1 - 2003/4/24
N2 - Intracellular free Ca2+ regulates diverse cellular processes, including membrane potential, neurotransmitter release, and gene expression. To examine the cellular mechanisms underlying the generation of circadian rhythms, nucleus-targeted and untargeted cDNAs encoding a Ca2+-sensitive fluorescent protein (cameleon) were transfected into organotypic cultures of mouse suprachiasmatic nucleus (SCN), the primary circadian pacemaker. Circadian rhythms in cytosolic but not nuclear Ca2+ concentration were observed in SCN neurons. The cytosolic Ca2+ rhythm period matched the circadian multiple-unit-activity (MUA)-rhythm period monitored using a multiple-electrode array, with a mean advance in phase of 4 hr. Tetrodotoxin blocked MUA, but not Ca2+ rhythms, while ryanodine damped both Ca2+ and MUA rhythms. These results demonstrate cytosolic Ca2+ rhythms regulated by the release of Ca2+ from ryanodine-sensitive stores in SCN neurons.
AB - Intracellular free Ca2+ regulates diverse cellular processes, including membrane potential, neurotransmitter release, and gene expression. To examine the cellular mechanisms underlying the generation of circadian rhythms, nucleus-targeted and untargeted cDNAs encoding a Ca2+-sensitive fluorescent protein (cameleon) were transfected into organotypic cultures of mouse suprachiasmatic nucleus (SCN), the primary circadian pacemaker. Circadian rhythms in cytosolic but not nuclear Ca2+ concentration were observed in SCN neurons. The cytosolic Ca2+ rhythm period matched the circadian multiple-unit-activity (MUA)-rhythm period monitored using a multiple-electrode array, with a mean advance in phase of 4 hr. Tetrodotoxin blocked MUA, but not Ca2+ rhythms, while ryanodine damped both Ca2+ and MUA rhythms. These results demonstrate cytosolic Ca2+ rhythms regulated by the release of Ca2+ from ryanodine-sensitive stores in SCN neurons.
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U2 - 10.1016/S0896-6273(03)00164-8
DO - 10.1016/S0896-6273(03)00164-8
M3 - Article
C2 - 12718859
AN - SCOPUS:0037688038
SN - 0896-6273
VL - 38
SP - 253
EP - 263
JO - Neuron
JF - Neuron
IS - 2
ER -