Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons

Masayuki Ikeda, Takashi Sugiyama, Christopher S. Wallace, Heinrich S. Gompf, Tohru Yoshioka, Atsushi Miyawaki, Charles Allen

Research output: Contribution to journalArticle

190 Citations (Scopus)

Abstract

Intracellular free Ca2+ regulates diverse cellular processes, including membrane potential, neurotransmitter release, and gene expression. To examine the cellular mechanisms underlying the generation of circadian rhythms, nucleus-targeted and untargeted cDNAs encoding a Ca2+-sensitive fluorescent protein (cameleon) were transfected into organotypic cultures of mouse suprachiasmatic nucleus (SCN), the primary circadian pacemaker. Circadian rhythms in cytosolic but not nuclear Ca2+ concentration were observed in SCN neurons. The cytosolic Ca2+ rhythm period matched the circadian multiple-unit-activity (MUA)-rhythm period monitored using a multiple-electrode array, with a mean advance in phase of 4 hr. Tetrodotoxin blocked MUA, but not Ca2+ rhythms, while ryanodine damped both Ca2+ and MUA rhythms. These results demonstrate cytosolic Ca2+ rhythms regulated by the release of Ca2+ from ryanodine-sensitive stores in SCN neurons.

Original languageEnglish (US)
Pages (from-to)253-263
Number of pages11
JournalNeuron
Volume38
Issue number2
DOIs
StatePublished - Apr 24 2003

Fingerprint

Suprachiasmatic Nucleus
Ryanodine
Circadian Rhythm
Neurons
Tetrodotoxin
Membrane Potentials
Neurotransmitter Agents
Electrodes
Complementary DNA
Gene Expression
Proteins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Ikeda, M., Sugiyama, T., Wallace, C. S., Gompf, H. S., Yoshioka, T., Miyawaki, A., & Allen, C. (2003). Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons. Neuron, 38(2), 253-263. https://doi.org/10.1016/S0896-6273(03)00164-8

Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons. / Ikeda, Masayuki; Sugiyama, Takashi; Wallace, Christopher S.; Gompf, Heinrich S.; Yoshioka, Tohru; Miyawaki, Atsushi; Allen, Charles.

In: Neuron, Vol. 38, No. 2, 24.04.2003, p. 253-263.

Research output: Contribution to journalArticle

Ikeda, M, Sugiyama, T, Wallace, CS, Gompf, HS, Yoshioka, T, Miyawaki, A & Allen, C 2003, 'Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons', Neuron, vol. 38, no. 2, pp. 253-263. https://doi.org/10.1016/S0896-6273(03)00164-8
Ikeda M, Sugiyama T, Wallace CS, Gompf HS, Yoshioka T, Miyawaki A et al. Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons. Neuron. 2003 Apr 24;38(2):253-263. https://doi.org/10.1016/S0896-6273(03)00164-8
Ikeda, Masayuki ; Sugiyama, Takashi ; Wallace, Christopher S. ; Gompf, Heinrich S. ; Yoshioka, Tohru ; Miyawaki, Atsushi ; Allen, Charles. / Circadian dynamics of cytosolic and nuclear Ca2+ in single suprachiasmatic nucleus neurons. In: Neuron. 2003 ; Vol. 38, No. 2. pp. 253-263.
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