Abstract
Circadian disruptions impair reproductive health in human populations and in animal models. We tested the hypothesis that mistimed food, a common disruptive feature of shift work, impairs reproductive success in mice. Male and female mPer2Luc mice on a C57BL/6 background were fed during the light or dark phase in two experiments. Food-induced internal misalignment of the liver clock was verified by in vivo bioluminescence in anesthetized mice in both experiments. In Experiment 1, food-restricted pairs were monitored for litters for 18 weeks. In the light-fed group, birth of the first litter was significantly delayed, and total reproductive output was significantly reduced by 38%. In Experiment 2, estrous cycling was monitored for 3 weeks, and then after pairing, copulatory plugs, pregnancy, litter sizes, and uterine implantation sites were measured. Fewer light-fed females birthed litters (25% versus 73%). This was attributable to a difference in behavior as mating success was significantly reduced in light-fed mice: 42% were observed with a copulatory plug compared to 82% for dark-fed mice. The proportion of mice displaying uterine implantation sites was the same as the proportion observed with copulatory plugs, suggesting no deficit in initiating pregnancy after mating. Estrous cycling and pregnancy maintenance did not differ between the groups. We conclude that mistimed feeding inhibits reproduction in mice by reducing successful mating behavior.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 177-184 |
| Number of pages | 8 |
| Journal | Hormones and Behavior |
| Volume | 105 |
| DOIs | |
| State | Published - Sep 1 2018 |
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Keywords
- Circadian clock
- Copulatory plug
- Estrous cycle
- Fertility
- Food restriction
- Pregnancy
- Reproduction
- Shift work
ASJC Scopus subject areas
- Endocrinology
- Endocrine and Autonomic Systems
- Behavioral Neuroscience
Cite this
Circadian disruption of food availability significantly reduces reproductive success in mice. / Swamy, Shivam; Xie, Xiaobin; Kukino, Ayaka; Calcagno, Haley E.; Lasarev, Michael R.; Park, Jin Ho; Butler, Matthew.
In: Hormones and Behavior, Vol. 105, 01.09.2018, p. 177-184.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Circadian disruption of food availability significantly reduces reproductive success in mice
AU - Swamy, Shivam
AU - Xie, Xiaobin
AU - Kukino, Ayaka
AU - Calcagno, Haley E.
AU - Lasarev, Michael R.
AU - Park, Jin Ho
AU - Butler, Matthew
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Circadian disruptions impair reproductive health in human populations and in animal models. We tested the hypothesis that mistimed food, a common disruptive feature of shift work, impairs reproductive success in mice. Male and female mPer2Luc mice on a C57BL/6 background were fed during the light or dark phase in two experiments. Food-induced internal misalignment of the liver clock was verified by in vivo bioluminescence in anesthetized mice in both experiments. In Experiment 1, food-restricted pairs were monitored for litters for 18 weeks. In the light-fed group, birth of the first litter was significantly delayed, and total reproductive output was significantly reduced by 38%. In Experiment 2, estrous cycling was monitored for 3 weeks, and then after pairing, copulatory plugs, pregnancy, litter sizes, and uterine implantation sites were measured. Fewer light-fed females birthed litters (25% versus 73%). This was attributable to a difference in behavior as mating success was significantly reduced in light-fed mice: 42% were observed with a copulatory plug compared to 82% for dark-fed mice. The proportion of mice displaying uterine implantation sites was the same as the proportion observed with copulatory plugs, suggesting no deficit in initiating pregnancy after mating. Estrous cycling and pregnancy maintenance did not differ between the groups. We conclude that mistimed feeding inhibits reproduction in mice by reducing successful mating behavior.
AB - Circadian disruptions impair reproductive health in human populations and in animal models. We tested the hypothesis that mistimed food, a common disruptive feature of shift work, impairs reproductive success in mice. Male and female mPer2Luc mice on a C57BL/6 background were fed during the light or dark phase in two experiments. Food-induced internal misalignment of the liver clock was verified by in vivo bioluminescence in anesthetized mice in both experiments. In Experiment 1, food-restricted pairs were monitored for litters for 18 weeks. In the light-fed group, birth of the first litter was significantly delayed, and total reproductive output was significantly reduced by 38%. In Experiment 2, estrous cycling was monitored for 3 weeks, and then after pairing, copulatory plugs, pregnancy, litter sizes, and uterine implantation sites were measured. Fewer light-fed females birthed litters (25% versus 73%). This was attributable to a difference in behavior as mating success was significantly reduced in light-fed mice: 42% were observed with a copulatory plug compared to 82% for dark-fed mice. The proportion of mice displaying uterine implantation sites was the same as the proportion observed with copulatory plugs, suggesting no deficit in initiating pregnancy after mating. Estrous cycling and pregnancy maintenance did not differ between the groups. We conclude that mistimed feeding inhibits reproduction in mice by reducing successful mating behavior.
KW - Circadian clock
KW - Copulatory plug
KW - Estrous cycle
KW - Fertility
KW - Food restriction
KW - Pregnancy
KW - Reproduction
KW - Shift work
UR - http://www.scopus.com/inward/record.url?scp=85052899875&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85052899875&partnerID=8YFLogxK
U2 - 10.1016/j.yhbeh.2018.07.006
DO - 10.1016/j.yhbeh.2018.07.006
M3 - Article
C2 - 30031683
AN - SCOPUS:85052899875
VL - 105
SP - 177
EP - 184
JO - Hormones and Behavior
JF - Hormones and Behavior
SN - 0018-506X
ER -