Circadian disruption of food availability significantly reduces reproductive success in mice

Shivam Swamy, Xiaobin Xie, Ayaka Kukino, Haley E. Calcagno, Michael R. Lasarev, Jin Ho Park, Matthew Butler

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Circadian disruptions impair reproductive health in human populations and in animal models. We tested the hypothesis that mistimed food, a common disruptive feature of shift work, impairs reproductive success in mice. Male and female mPer2Luc mice on a C57BL/6 background were fed during the light or dark phase in two experiments. Food-induced internal misalignment of the liver clock was verified by in vivo bioluminescence in anesthetized mice in both experiments. In Experiment 1, food-restricted pairs were monitored for litters for 18 weeks. In the light-fed group, birth of the first litter was significantly delayed, and total reproductive output was significantly reduced by 38%. In Experiment 2, estrous cycling was monitored for 3 weeks, and then after pairing, copulatory plugs, pregnancy, litter sizes, and uterine implantation sites were measured. Fewer light-fed females birthed litters (25% versus 73%). This was attributable to a difference in behavior as mating success was significantly reduced in light-fed mice: 42% were observed with a copulatory plug compared to 82% for dark-fed mice. The proportion of mice displaying uterine implantation sites was the same as the proportion observed with copulatory plugs, suggesting no deficit in initiating pregnancy after mating. Estrous cycling and pregnancy maintenance did not differ between the groups. We conclude that mistimed feeding inhibits reproduction in mice by reducing successful mating behavior.

Original languageEnglish (US)
Pages (from-to)177-184
Number of pages8
JournalHormones and Behavior
Volume105
DOIs
StatePublished - Sep 1 2018

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Food
Light
Pregnancy Maintenance
Pregnancy
Litter Size
Birth Order
Reproductive Health
Reproduction
Animal Models
Liver
Population

Keywords

  • Circadian clock
  • Copulatory plug
  • Estrous cycle
  • Fertility
  • Food restriction
  • Pregnancy
  • Reproduction
  • Shift work

ASJC Scopus subject areas

  • Endocrinology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

Cite this

Circadian disruption of food availability significantly reduces reproductive success in mice. / Swamy, Shivam; Xie, Xiaobin; Kukino, Ayaka; Calcagno, Haley E.; Lasarev, Michael R.; Park, Jin Ho; Butler, Matthew.

In: Hormones and Behavior, Vol. 105, 01.09.2018, p. 177-184.

Research output: Contribution to journalArticle

Swamy, Shivam ; Xie, Xiaobin ; Kukino, Ayaka ; Calcagno, Haley E. ; Lasarev, Michael R. ; Park, Jin Ho ; Butler, Matthew. / Circadian disruption of food availability significantly reduces reproductive success in mice. In: Hormones and Behavior. 2018 ; Vol. 105. pp. 177-184.
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