Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages

Suhas Sureshchandra, Cara Stull, Brian Jin Kee Ligh, Selene Bich Nguyen, Kathleen (Kathy) Grant, Ilhem Messaoudi

    Research output: Contribution to journalArticle

    Abstract

    Background: Chronic heavy alcohol drinking (CHD) leads to significant organ damage, increased susceptibility to infections, and delayed wound healing. These adverse outcomes are believed to be mediated by alterations in the function of myeloid cells; however, the mechanisms underlying these changes are poorly understood. Methods: We determined the impact of CHD on the phenotype of splenic macrophages using flow cytometry. Changes in functional responses to LPS were measured using luminex and RNA-Seq. Finally, alterations in chromatin accessibility were uncovered using ATAC-Seq. Findings: A history of CHD led to increased frequency of splenic macrophages that exhibited a heightened activation state at resting. Additionally, splenic macrophages from CHD animals generated a larger inflammatory response to LPS, both at protein and gene expression levels. Finally, CHD resulted in increased levels of H3K4me3, a histone mark of active promoters, as well as chromatin accessibility at promoters and intergenic regions that regulate inflammatory responses. Interpretation: These findings suggest that a history of CHD alters the immune fitness of tissue-resident macrophages via epigenetic mechanisms. Fund: National Institute on Alcohol Abuse and Alcoholism (NIAAA), National Institutes of Health (NIH) - R24AA019431, U01 AA13641, U01 AA13510, R21AA021947, and R21AA025839.

    Original languageEnglish (US)
    JournalEBioMedicine
    DOIs
    StatePublished - Jan 1 2019

    Fingerprint

    Macrophages
    Epigenomics
    Alcohol Drinking
    Drinking
    Alcohols
    Chromatin
    National Institute on Alcohol Abuse and Alcoholism (U.S.)
    Histone Code
    Intergenic DNA
    National Institutes of Health (U.S.)
    Myeloid Cells
    Flow cytometry
    Genetic Promoter Regions
    Wound Healing
    Drive
    Gene expression
    Flow Cytometry
    Histones
    RNA
    Animals

    Keywords

    • Chromatin accessibility
    • Chronic heavy drinking
    • Ethanol
    • LPS
    • Rhesus macaques
    • Splenic macrophages

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)

    Cite this

    Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages. / Sureshchandra, Suhas; Stull, Cara; Ligh, Brian Jin Kee; Nguyen, Selene Bich; Grant, Kathleen (Kathy); Messaoudi, Ilhem.

    In: EBioMedicine, 01.01.2019.

    Research output: Contribution to journalArticle

    Sureshchandra, Suhas ; Stull, Cara ; Ligh, Brian Jin Kee ; Nguyen, Selene Bich ; Grant, Kathleen (Kathy) ; Messaoudi, Ilhem. / Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages. In: EBioMedicine. 2019.
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