Chronic functional ethanol tolerance in mice influenced by body temperature during acquisition

R. L. Alkana, M. Benjanian, P. J. Syapin, Deborah (Deb) Finn

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Previous studies have found that body temperature during intoxication influences brain sensitivity to ethanol with the sensitivity being less at cool than at warm body temperatures. If this effect of temperature reflects alterations in the acute membrane perturbing action of ethanol, as suggested by in vitro studies, then body temperature reduction (hypothermia) during tolerance acquisition should reduce the effectiveness of a given ethanol concentration and, in turn, should reduce the development of chronic functional ethanol tolerance. To test this hypothesis, adult drug-naive C57BL/6J mice were injected i.p. once daily for five days with 3.6 g/kg ethanol (20% w/v) and were exposed to 34°C or 25°C for five hours following injection. On day 6, both ethanol acquisition groups and naive mice were injected i.p. with 4.0 g/kg ethanol and exposed to 25°C. During acquisition, the group exposed to 34°C had significantly higher body temperatures than the mice exposed to 25°C, and there were no statistically significant differences in blood ethanol concentrations between treatment conditions. The extent of tolerance on day 6, measured by sleep-times and wake-up blood and brain ethanol concentrations versus naive mice, was significantly greater in the 34°C acquisition group than in the 25°C acquisition group. The results demonstrate that body temperature influences tolerance development in the manner predicted by membrane perturbation theories of anesthesia and adaptation based tolerance theories.

Original languageEnglish (US)
Pages (from-to)413-420
Number of pages8
JournalLife Sciences
Volume41
Issue number4
DOIs
StatePublished - Jul 27 1987
Externally publishedYes

Fingerprint

Body Temperature
Ethanol
Temperature
Brain
Blood
Hypothermia
Membranes
Inbred C57BL Mouse
Sleep
Anesthesia
Injections
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Pharmacology

Cite this

Chronic functional ethanol tolerance in mice influenced by body temperature during acquisition. / Alkana, R. L.; Benjanian, M.; Syapin, P. J.; Finn, Deborah (Deb).

In: Life Sciences, Vol. 41, No. 4, 27.07.1987, p. 413-420.

Research output: Contribution to journalArticle

Alkana, R. L. ; Benjanian, M. ; Syapin, P. J. ; Finn, Deborah (Deb). / Chronic functional ethanol tolerance in mice influenced by body temperature during acquisition. In: Life Sciences. 1987 ; Vol. 41, No. 4. pp. 413-420.
@article{9f5133b0a7a1487e85e399f65caff426,
title = "Chronic functional ethanol tolerance in mice influenced by body temperature during acquisition",
abstract = "Previous studies have found that body temperature during intoxication influences brain sensitivity to ethanol with the sensitivity being less at cool than at warm body temperatures. If this effect of temperature reflects alterations in the acute membrane perturbing action of ethanol, as suggested by in vitro studies, then body temperature reduction (hypothermia) during tolerance acquisition should reduce the effectiveness of a given ethanol concentration and, in turn, should reduce the development of chronic functional ethanol tolerance. To test this hypothesis, adult drug-naive C57BL/6J mice were injected i.p. once daily for five days with 3.6 g/kg ethanol (20{\%} w/v) and were exposed to 34°C or 25°C for five hours following injection. On day 6, both ethanol acquisition groups and naive mice were injected i.p. with 4.0 g/kg ethanol and exposed to 25°C. During acquisition, the group exposed to 34°C had significantly higher body temperatures than the mice exposed to 25°C, and there were no statistically significant differences in blood ethanol concentrations between treatment conditions. The extent of tolerance on day 6, measured by sleep-times and wake-up blood and brain ethanol concentrations versus naive mice, was significantly greater in the 34°C acquisition group than in the 25°C acquisition group. The results demonstrate that body temperature influences tolerance development in the manner predicted by membrane perturbation theories of anesthesia and adaptation based tolerance theories.",
author = "Alkana, {R. L.} and M. Benjanian and Syapin, {P. J.} and Finn, {Deborah (Deb)}",
year = "1987",
month = "7",
day = "27",
doi = "10.1016/0024-3205(87)90216-5",
language = "English (US)",
volume = "41",
pages = "413--420",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Chronic functional ethanol tolerance in mice influenced by body temperature during acquisition

AU - Alkana, R. L.

AU - Benjanian, M.

AU - Syapin, P. J.

AU - Finn, Deborah (Deb)

PY - 1987/7/27

Y1 - 1987/7/27

N2 - Previous studies have found that body temperature during intoxication influences brain sensitivity to ethanol with the sensitivity being less at cool than at warm body temperatures. If this effect of temperature reflects alterations in the acute membrane perturbing action of ethanol, as suggested by in vitro studies, then body temperature reduction (hypothermia) during tolerance acquisition should reduce the effectiveness of a given ethanol concentration and, in turn, should reduce the development of chronic functional ethanol tolerance. To test this hypothesis, adult drug-naive C57BL/6J mice were injected i.p. once daily for five days with 3.6 g/kg ethanol (20% w/v) and were exposed to 34°C or 25°C for five hours following injection. On day 6, both ethanol acquisition groups and naive mice were injected i.p. with 4.0 g/kg ethanol and exposed to 25°C. During acquisition, the group exposed to 34°C had significantly higher body temperatures than the mice exposed to 25°C, and there were no statistically significant differences in blood ethanol concentrations between treatment conditions. The extent of tolerance on day 6, measured by sleep-times and wake-up blood and brain ethanol concentrations versus naive mice, was significantly greater in the 34°C acquisition group than in the 25°C acquisition group. The results demonstrate that body temperature influences tolerance development in the manner predicted by membrane perturbation theories of anesthesia and adaptation based tolerance theories.

AB - Previous studies have found that body temperature during intoxication influences brain sensitivity to ethanol with the sensitivity being less at cool than at warm body temperatures. If this effect of temperature reflects alterations in the acute membrane perturbing action of ethanol, as suggested by in vitro studies, then body temperature reduction (hypothermia) during tolerance acquisition should reduce the effectiveness of a given ethanol concentration and, in turn, should reduce the development of chronic functional ethanol tolerance. To test this hypothesis, adult drug-naive C57BL/6J mice were injected i.p. once daily for five days with 3.6 g/kg ethanol (20% w/v) and were exposed to 34°C or 25°C for five hours following injection. On day 6, both ethanol acquisition groups and naive mice were injected i.p. with 4.0 g/kg ethanol and exposed to 25°C. During acquisition, the group exposed to 34°C had significantly higher body temperatures than the mice exposed to 25°C, and there were no statistically significant differences in blood ethanol concentrations between treatment conditions. The extent of tolerance on day 6, measured by sleep-times and wake-up blood and brain ethanol concentrations versus naive mice, was significantly greater in the 34°C acquisition group than in the 25°C acquisition group. The results demonstrate that body temperature influences tolerance development in the manner predicted by membrane perturbation theories of anesthesia and adaptation based tolerance theories.

UR - http://www.scopus.com/inward/record.url?scp=0023203729&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023203729&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(87)90216-5

DO - 10.1016/0024-3205(87)90216-5

M3 - Article

C2 - 3600185

AN - SCOPUS:0023203729

VL - 41

SP - 413

EP - 420

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 4

ER -