TY - JOUR
T1 - Chronic exposure of the developing corpus luteum in monkeys to chorionic gonadotropin
T2 - Persistent progesterone production despite desensitization of adenylate cyclase
AU - Vandevoort, Catherine A.
AU - Stouffer, Richard L.
AU - Molskness, Theodore A.
AU - Ottobre, Joseph S.
PY - 1988/5/1
Y1 - 1988/5/1
N2 - The transient steroidogenic response of the macaque corpus luteum to chronic human CG (hCG) treatment beginning on days 9–10 of the luteal phase (i.e. stimulated early pregnancy) is associated with decreased numbers and affinity of available receptors for gonadotropin and homologous desensitization of adenylate cyclase. This study determined if similar changes in the receptor-adenylate cyclase system accompany the persistent steroidogenic response which occurs when hCG treatment begins earlier in the luteal phase. Female rhesus monkeys received increasing doses of hCG (15 up to 5760 LU) twice daily beginning 5–6 days after the midcycle LH surge. The levels of circulating progesterone increased (P < 0.05) within 24 h of initial hCG exposure and did not decrease throughout the 10- day regimen. The corpus luteum was removed after 0 (n = 8), 6 (n = 4), or 10 (n = 4) days of hCG treatment. Whereas the numbers of available [125I]hCG binding sites in luteal particulates remained unchanged by 10 days of hCG exposure, the dissociation constant (K > 0 for gonadotropin binding was greater than at day 0 (6.17 ± 1.41 vs. 0.91 ± 0.06 × 10-10 M, P < 0.05). Since the number of binding sites occupied by injected hCG increased with treatment (7.81 ± 1.55 fmol/mg wet wt at day 10), the total number (available + occupied) of gonadotropin receptors was 3-fold greater (P < 0.05) at day 10 than at day 0. Adenylate cyclase activity in luteal homogenates, assessed by conversion of [a-32P]ATP to [32P]cAMP, was stimulated on day 0 by hCG (2.7 ± 0.7 × control, at 250 nM hCG), prostaglandin E2 (2.5 + 0.5 × control, at 0.5 mM), and prostaglandin I2 (2.3 ± 0.5 × control at 0.5 mM) as well as forskolin (100 µM) and 5’- guanylyl-imidodiphosphate (50 µM). In contrast, cAMP production by day 6 of treatment was insensitive to hCG, but remained responsive to prostaglandin E2, prostaglandin I2, and nonhormonal activators. We conclude that CG treatment in the early luteal phase did not prevent the development of gonadotropin receptors to levels typically observed in the functional corpus luteum of the menstrual cycle. Also, many changes in the gonadotropin receptor-adenylate cyclase system in macaque luteal tissue were similar after CG treatment beginning on days 5–6 or days 9–10 of the luteal phase. Thus, these membrane events may not mediate the transitory response of the primate corpus luteum to CG in early pregnancy; indeed, luteal steroidogenesis can persist despite desensitization of adenylate cyclase to gonadotropin.
AB - The transient steroidogenic response of the macaque corpus luteum to chronic human CG (hCG) treatment beginning on days 9–10 of the luteal phase (i.e. stimulated early pregnancy) is associated with decreased numbers and affinity of available receptors for gonadotropin and homologous desensitization of adenylate cyclase. This study determined if similar changes in the receptor-adenylate cyclase system accompany the persistent steroidogenic response which occurs when hCG treatment begins earlier in the luteal phase. Female rhesus monkeys received increasing doses of hCG (15 up to 5760 LU) twice daily beginning 5–6 days after the midcycle LH surge. The levels of circulating progesterone increased (P < 0.05) within 24 h of initial hCG exposure and did not decrease throughout the 10- day regimen. The corpus luteum was removed after 0 (n = 8), 6 (n = 4), or 10 (n = 4) days of hCG treatment. Whereas the numbers of available [125I]hCG binding sites in luteal particulates remained unchanged by 10 days of hCG exposure, the dissociation constant (K > 0 for gonadotropin binding was greater than at day 0 (6.17 ± 1.41 vs. 0.91 ± 0.06 × 10-10 M, P < 0.05). Since the number of binding sites occupied by injected hCG increased with treatment (7.81 ± 1.55 fmol/mg wet wt at day 10), the total number (available + occupied) of gonadotropin receptors was 3-fold greater (P < 0.05) at day 10 than at day 0. Adenylate cyclase activity in luteal homogenates, assessed by conversion of [a-32P]ATP to [32P]cAMP, was stimulated on day 0 by hCG (2.7 ± 0.7 × control, at 250 nM hCG), prostaglandin E2 (2.5 + 0.5 × control, at 0.5 mM), and prostaglandin I2 (2.3 ± 0.5 × control at 0.5 mM) as well as forskolin (100 µM) and 5’- guanylyl-imidodiphosphate (50 µM). In contrast, cAMP production by day 6 of treatment was insensitive to hCG, but remained responsive to prostaglandin E2, prostaglandin I2, and nonhormonal activators. We conclude that CG treatment in the early luteal phase did not prevent the development of gonadotropin receptors to levels typically observed in the functional corpus luteum of the menstrual cycle. Also, many changes in the gonadotropin receptor-adenylate cyclase system in macaque luteal tissue were similar after CG treatment beginning on days 5–6 or days 9–10 of the luteal phase. Thus, these membrane events may not mediate the transitory response of the primate corpus luteum to CG in early pregnancy; indeed, luteal steroidogenesis can persist despite desensitization of adenylate cyclase to gonadotropin.
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U2 - 10.1210/endo-122-5-1876
DO - 10.1210/endo-122-5-1876
M3 - Article
C2 - 2834178
AN - SCOPUS:0023913949
SN - 0013-7227
VL - 122
SP - 1876
EP - 1882
JO - Endocrinology
JF - Endocrinology
IS - 5
ER -