Chronic ethanol consumption alters lamina propria leukocyte response to stimulation in a region-dependent manner

Tasha Barr, Sloan A. Lewis, Suhas Sureshchandra, Brianna Doratt, Kathleen (Kathy) Grant, Ilhem Messaoudi

    Research output: Contribution to journalArticle

    Abstract

    Chronic heavy alcohol consumption, also referred to as chronic heavy drinking (CHD), results in intestinal injury characterized by increased permeability, dysbiosis, nutrient malabsorption, potentially higher susceptibility to infection, and increased risk of colorectal cancer. However, our understanding of the mechanisms by which CHD results in intestinal damage remains incomplete. Here, we investigated the impact of chronic drinking on transcriptional and functional responses of lamina propria leukocytes (LPLs) isolated from the 4 major gut sections. Although no significant differences were detected between LPLs isolated from the ethanol and control groups at resting state within each major gut section, our analysis uncovered key regional differences in composition and function of LPLs independent of alcohol consumption. However, in response to phorbol myristate acetate and ionomycin, duodenal LPLs from ethanol-drinking animals generated a dampened response, whereas jejunal and ileal LPLs from ethanol-drinking animals produced a heightened response. Transcriptional responses following stimulation were pronounced in ileal and duodenal LPLs from the ethanol-drinking group but less evident in jejunal and colonic LPLs compared with controls, suggesting a more significant impact of alcohol on these gut regions. The altered intestinal LPL function detected in our study reveals remarkable region specificity and novel insight into potential mechanisms of intestinal injury associated with CHD.-Barr, T., Lewis, S. A., Sureshchandra, S., Doratt, B., Grant, K. A., Messaoudi, I. Chronic ethanol consumption alters lamina propria leukocyte response to stimulation in a region-dependent manner.

    Original languageEnglish (US)
    Pages (from-to)7767-7777
    Number of pages11
    JournalFASEB journal : official publication of the Federation of American Societies for Experimental Biology
    Volume33
    Issue number6
    DOIs
    StatePublished - Jun 1 2019

    Fingerprint

    Mucous Membrane
    Leukocytes
    Ethanol
    Drinking
    Alcohols
    Alcohol Drinking
    Animals
    Dysbiosis
    Ionomycin
    Wounds and Injuries
    Tetradecanoylphorbol Acetate
    Nutrients
    Colorectal Neoplasms
    Permeability
    Food
    Control Groups
    Infection
    Chemical analysis

    Keywords

    • alcohol and immunity
    • gastrointestinal tract
    • mucosal immunology
    • nonhuman primate

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Genetics

    Cite this

    Chronic ethanol consumption alters lamina propria leukocyte response to stimulation in a region-dependent manner. / Barr, Tasha; Lewis, Sloan A.; Sureshchandra, Suhas; Doratt, Brianna; Grant, Kathleen (Kathy); Messaoudi, Ilhem.

    In: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 33, No. 6, 01.06.2019, p. 7767-7777.

    Research output: Contribution to journalArticle

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