Chronic anemic hypoxemia attenuates glucose-stimulated insulin secretion in fetal sheep

Joshua S. Benjamin, Christine B. Culpepper, Laura D. Brown, Stephanie R. Wesolowski, Sonnet Jonker, Melissa A. Davis, Sean W. Limesand, Randall B. Wilkening, William W. Hay, Paul J. Rozance

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Fetal insulin secretion is inhibited by acute hypoxemia. The relationship between prolonged hypoxemia and insulin secretion, however, is less well defined. To test the hypothesis that prolonged fetal hypoxemia impairs insulin secretion, studies were performed in sheep fetuses that were bled to anemic conditions for 9 ± 0 days (anemic, n = 19) and compared with control fetuses (n = 15). Arterial hematocrit and oxygen content were 34% and 52% lower, respectively, in anemic vs. control fetuses (P < 0.0001). Plasma glucose concentrations were 21% higher in the anemic group (P < 0.05). Plasma norepinephrine and cortisol concentrations increased 70% in the anemic group (P < 0.05). Glucose-, arginine-, and leucine-stimulated insulin secretion all were lower (P < 0.05) in anemic fetuses. No differences in pancreatic islet size or β-cell mass were found. In vitro, isolated islets from anemic fetuses secreted insulin in response to glucose and leucine as well as control fetal islets. These findings indicate a functional islet defect in anemic fetuses, which likely involves direct effects of low oxygen and/or increased norepinephrine on insulin release. In pregnancies complicated by chronic fetal hypoxemia, increasing fetal oxygen concentrations may improve insulin secretion.

    Original languageEnglish (US)
    Pages (from-to)R492-R500
    JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
    Volume312
    Issue number4
    DOIs
    StatePublished - Apr 1 2017

    Fingerprint

    Sheep
    Insulin
    Fetus
    Glucose
    Oxygen
    Leucine
    Norepinephrine
    Hypoxia
    Islets of Langerhans
    Cell Size
    Hematocrit
    Arginine
    Hydrocortisone
    Pregnancy

    Keywords

    • Fetus
    • Islet
    • Oxygen
    • Pancreas
    • β-cell

    ASJC Scopus subject areas

    • Physiology
    • Physiology (medical)

    Cite this

    Chronic anemic hypoxemia attenuates glucose-stimulated insulin secretion in fetal sheep. / Benjamin, Joshua S.; Culpepper, Christine B.; Brown, Laura D.; Wesolowski, Stephanie R.; Jonker, Sonnet; Davis, Melissa A.; Limesand, Sean W.; Wilkening, Randall B.; Hay, William W.; Rozance, Paul J.

    In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 312, No. 4, 01.04.2017, p. R492-R500.

    Research output: Contribution to journalArticle

    Benjamin, JS, Culpepper, CB, Brown, LD, Wesolowski, SR, Jonker, S, Davis, MA, Limesand, SW, Wilkening, RB, Hay, WW & Rozance, PJ 2017, 'Chronic anemic hypoxemia attenuates glucose-stimulated insulin secretion in fetal sheep', American Journal of Physiology - Regulatory Integrative and Comparative Physiology, vol. 312, no. 4, pp. R492-R500. https://doi.org/10.1152/ajpregu.00484.2016
    Benjamin, Joshua S. ; Culpepper, Christine B. ; Brown, Laura D. ; Wesolowski, Stephanie R. ; Jonker, Sonnet ; Davis, Melissa A. ; Limesand, Sean W. ; Wilkening, Randall B. ; Hay, William W. ; Rozance, Paul J. / Chronic anemic hypoxemia attenuates glucose-stimulated insulin secretion in fetal sheep. In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology. 2017 ; Vol. 312, No. 4. pp. R492-R500.
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