Chromosome measurement and sorting by flow systems

J. W. Gray, A. V. Carrano, L. L. Steinmetz, M. A. Van Dilla, D. H. Moore IInd, B. H. Mayall, M. L. Mendelsohn

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Abstract

A flow microfluorometer was used to measure metaphase chromosomes in suspension at rates up to 100,000 per min. Chromosomes from cells of the Chinese hamster M3 1 cell line were isolated, stained for DNA with the fluorescent dye ethidium bromide, and analyzed for DNA content. Nine distinct peaks were resolved that correspond well with independent chromosomal DNA measurements made with a high resolution scanning cytophotometer. Chromosomes were sorted from each peak by an electronic cell sorter. Visual examination of each fraction indicated the purity of the sorted chromosomes. The novel technology allows separation of purified populations of individual chromosomes suitable for biochemical and biological characterizations.

Original languageEnglish (US)
Pages (from-to)1231-1234
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number4
DOIs
StatePublished - 1975

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    Gray, J. W., Carrano, A. V., Steinmetz, L. L., Van Dilla, M. A., Moore IInd, D. H., Mayall, B. H., & Mendelsohn, M. L. (1975). Chromosome measurement and sorting by flow systems. Proceedings of the National Academy of Sciences of the United States of America, 72(4), 1231-1234. https://doi.org/10.1073/pnas.72.4.1231