TY - JOUR
T1 - Chromosomal localization of three human D5 dopamine receptor genes
AU - Grandy, David K.
AU - Allen, Lee J.
AU - Zhang, Yuan
AU - Magenis, R. Ellen
AU - Civelli, Olivier
N1 - Funding Information:
We thank Qun-Yong Zhou for the primers 545 and 546 and Drs. David Cox, Nicholas Dracopoli, Michael Litt, Richard Meyers, and Vikram Sharma for the generous sharing of genomic DNAs. We also thank Dr. Litt for his critical reading of the manuscript and June Shiigi and Scott Thompson for preparing the illustrations. This work was supported by grants from the Scottish Rite Foundation (D.K.G., O.C.) and NIH Grant MH45614 (O.C.).
PY - 1992/8
Y1 - 1992/8
N2 - It is currently thought that genetic predisposition to imbalances in dopaminergic transmission may underlie several neurological disorders, including schizophrenia, manic depression, Tourette syndrome, Parkinson disease, Huntington disease, and alcohol abuse. Originally two receptors, D1 and D2, were thought to account for all of the pharmacological actions of dopamine. However, through homology screening three additional genes, D3, D4, and D5, and two pseudogenes closely related to D5 have been characterized. To begin our genomic and evolutionary analyses of the human D5 dopamine receptor gene and its two pseudogenes, we have mapped each of them to their respective chromosomes. By combining in situ hybridization results with sequence analysis of PCR products from microdissected chromosomes, somatic cell hybrids, and radiation hybrids, we have assigned DRD5 (the locus containing the functional human D5 receptor gene) to chromosome 4p16.1, DRD5P1 (the locus containing D5 pseudogene 1) to chromosome 2p11.1-p11.2, and DRD5P2 (the locus of D5 pseudogene 2) to chromosome 1q21.1.
AB - It is currently thought that genetic predisposition to imbalances in dopaminergic transmission may underlie several neurological disorders, including schizophrenia, manic depression, Tourette syndrome, Parkinson disease, Huntington disease, and alcohol abuse. Originally two receptors, D1 and D2, were thought to account for all of the pharmacological actions of dopamine. However, through homology screening three additional genes, D3, D4, and D5, and two pseudogenes closely related to D5 have been characterized. To begin our genomic and evolutionary analyses of the human D5 dopamine receptor gene and its two pseudogenes, we have mapped each of them to their respective chromosomes. By combining in situ hybridization results with sequence analysis of PCR products from microdissected chromosomes, somatic cell hybrids, and radiation hybrids, we have assigned DRD5 (the locus containing the functional human D5 receptor gene) to chromosome 4p16.1, DRD5P1 (the locus containing D5 pseudogene 1) to chromosome 2p11.1-p11.2, and DRD5P2 (the locus of D5 pseudogene 2) to chromosome 1q21.1.
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U2 - 10.1016/0888-7543(92)90009-H
DO - 10.1016/0888-7543(92)90009-H
M3 - Article
C2 - 1387108
AN - SCOPUS:0026742787
SN - 0888-7543
VL - 13
SP - 968
EP - 973
JO - Genomics
JF - Genomics
IS - 4
ER -