Riparazione dei difetti a tutto spessore della cartilagine articolare con cellule condroprogenitrici

Translated title of the contribution: Chondroprogenitor cell repair of full thickness defects of articular cartilage

V. M. Goldberg, L. Solchaga, Jung Yoo, Brian Johnstone, A. I. Caplan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Articular cartilage is a unique tissue since it is avascular, aneural and has a limited capacity to repair. The repair of cartilage depends upon access to progenitor cells present in the underlying bone marrow. The studies presented here emphasize cell-based methods of repair of articular cartilage defects using these cells. Chondroprogenitor cells were used in combination with either Type I collagen gels or a polymer of benzyl-derivatized hyaluronic acid as a cell carrier. The repair of 3 mm full thickness articular cartilage defects in the medial femoral condyle of young adult New Zealand rabbits was studied. Chondroprogenitor cells were isolated from autologous bone marrow, purified and culture expanded and then loaded either into Type I collagen gels or the polymer of hyaluronic acid. Animals were killed four and twelve weeks after surgery and the repair of the defect histologically evaluated. Both cell carrier implants resulted in significant repair of the defect by twelve weeks after surgery. The repair tissue resembled hyalin cartilage with significant production of toluidine blue positive extracellular matrix. Subchondral bone remodelled and the tidemark was reconstituted in most defects. However, incomplete integration of the repair tissue to the host consistently was seen. The surface was roughened and irregular twelve weeks after implantation. These studies suggest that tissue engineered implants utilizing chondroprogenitor cells and biological carriers can effectively repair full thickness defects of articular surfaces.

Original languageItalian
Pages (from-to)81-89
Number of pages9
JournalJournal of Sports Traumatology and Related Research
Volume20
Issue number2
StatePublished - 1998

Fingerprint

Articular Cartilage
Hyaluronic Acid
Collagen Type I
Cartilage
Polymers
Bone Marrow
Gels
Bone and Bones
Tolonium Chloride
Hyalin
Thigh
Extracellular Matrix
Young Adult
Stem Cells
Joints
Rabbits

Keywords

  • Cartilage defect
  • Progenitor cell
  • Repair

ASJC Scopus subject areas

  • Surgery
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Riparazione dei difetti a tutto spessore della cartilagine articolare con cellule condroprogenitrici. / Goldberg, V. M.; Solchaga, L.; Yoo, Jung; Johnstone, Brian; Caplan, A. I.

In: Journal of Sports Traumatology and Related Research, Vol. 20, No. 2, 1998, p. 81-89.

Research output: Contribution to journalArticle

@article{155e2ffd048e4eaabce06d8f4a7ef8fb,
title = "Riparazione dei difetti a tutto spessore della cartilagine articolare con cellule condroprogenitrici",
abstract = "Articular cartilage is a unique tissue since it is avascular, aneural and has a limited capacity to repair. The repair of cartilage depends upon access to progenitor cells present in the underlying bone marrow. The studies presented here emphasize cell-based methods of repair of articular cartilage defects using these cells. Chondroprogenitor cells were used in combination with either Type I collagen gels or a polymer of benzyl-derivatized hyaluronic acid as a cell carrier. The repair of 3 mm full thickness articular cartilage defects in the medial femoral condyle of young adult New Zealand rabbits was studied. Chondroprogenitor cells were isolated from autologous bone marrow, purified and culture expanded and then loaded either into Type I collagen gels or the polymer of hyaluronic acid. Animals were killed four and twelve weeks after surgery and the repair of the defect histologically evaluated. Both cell carrier implants resulted in significant repair of the defect by twelve weeks after surgery. The repair tissue resembled hyalin cartilage with significant production of toluidine blue positive extracellular matrix. Subchondral bone remodelled and the tidemark was reconstituted in most defects. However, incomplete integration of the repair tissue to the host consistently was seen. The surface was roughened and irregular twelve weeks after implantation. These studies suggest that tissue engineered implants utilizing chondroprogenitor cells and biological carriers can effectively repair full thickness defects of articular surfaces.",
keywords = "Cartilage defect, Progenitor cell, Repair",
author = "Goldberg, {V. M.} and L. Solchaga and Jung Yoo and Brian Johnstone and Caplan, {A. I.}",
year = "1998",
language = "Italian",
volume = "20",
pages = "81--89",
journal = "Journal of Sports Traumatology and Related Research",
issn = "1120-3137",
publisher = "Editrice Kurtis s.r.l.",
number = "2",

}

TY - JOUR

T1 - Riparazione dei difetti a tutto spessore della cartilagine articolare con cellule condroprogenitrici

AU - Goldberg, V. M.

AU - Solchaga, L.

AU - Yoo, Jung

AU - Johnstone, Brian

AU - Caplan, A. I.

PY - 1998

Y1 - 1998

N2 - Articular cartilage is a unique tissue since it is avascular, aneural and has a limited capacity to repair. The repair of cartilage depends upon access to progenitor cells present in the underlying bone marrow. The studies presented here emphasize cell-based methods of repair of articular cartilage defects using these cells. Chondroprogenitor cells were used in combination with either Type I collagen gels or a polymer of benzyl-derivatized hyaluronic acid as a cell carrier. The repair of 3 mm full thickness articular cartilage defects in the medial femoral condyle of young adult New Zealand rabbits was studied. Chondroprogenitor cells were isolated from autologous bone marrow, purified and culture expanded and then loaded either into Type I collagen gels or the polymer of hyaluronic acid. Animals were killed four and twelve weeks after surgery and the repair of the defect histologically evaluated. Both cell carrier implants resulted in significant repair of the defect by twelve weeks after surgery. The repair tissue resembled hyalin cartilage with significant production of toluidine blue positive extracellular matrix. Subchondral bone remodelled and the tidemark was reconstituted in most defects. However, incomplete integration of the repair tissue to the host consistently was seen. The surface was roughened and irregular twelve weeks after implantation. These studies suggest that tissue engineered implants utilizing chondroprogenitor cells and biological carriers can effectively repair full thickness defects of articular surfaces.

AB - Articular cartilage is a unique tissue since it is avascular, aneural and has a limited capacity to repair. The repair of cartilage depends upon access to progenitor cells present in the underlying bone marrow. The studies presented here emphasize cell-based methods of repair of articular cartilage defects using these cells. Chondroprogenitor cells were used in combination with either Type I collagen gels or a polymer of benzyl-derivatized hyaluronic acid as a cell carrier. The repair of 3 mm full thickness articular cartilage defects in the medial femoral condyle of young adult New Zealand rabbits was studied. Chondroprogenitor cells were isolated from autologous bone marrow, purified and culture expanded and then loaded either into Type I collagen gels or the polymer of hyaluronic acid. Animals were killed four and twelve weeks after surgery and the repair of the defect histologically evaluated. Both cell carrier implants resulted in significant repair of the defect by twelve weeks after surgery. The repair tissue resembled hyalin cartilage with significant production of toluidine blue positive extracellular matrix. Subchondral bone remodelled and the tidemark was reconstituted in most defects. However, incomplete integration of the repair tissue to the host consistently was seen. The surface was roughened and irregular twelve weeks after implantation. These studies suggest that tissue engineered implants utilizing chondroprogenitor cells and biological carriers can effectively repair full thickness defects of articular surfaces.

KW - Cartilage defect

KW - Progenitor cell

KW - Repair

UR - http://www.scopus.com/inward/record.url?scp=0031734946&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031734946&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0031734946

VL - 20

SP - 81

EP - 89

JO - Journal of Sports Traumatology and Related Research

JF - Journal of Sports Traumatology and Related Research

SN - 1120-3137

IS - 2

ER -