Chondrogenic and gliogenic subpopulations of neural crest play distinct roles during the assembly of epibranchial ganglia

Maya D. Culbertson, Zachary R. Lewis, Alex Nechiporuk

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In vertebrates, the sensory neurons of the epibranchial (EB) ganglia transmit somatosensory signals from the periphery to the CNS. These ganglia are formed during embryogenesis by the convergence and condensation of two distinct populations of precursors: placode-derived neuroblasts and neural crest- (NC) derived glial precursors. In addition to the gliogenic crest, chondrogenic NC migrates into the pharyngeal arches, which lie in close proximity to the EB placodes and ganglia. Here, we examine the respective roles of these two distinct NC-derived populations during development of the EB ganglia using zebrafish morphant and mutants that lack one or both of these NC populations. Our analyses of mutant and morphant zebrafish that exhibit deficiencies in chondrogenic NC at early stages reveal a distinct requirement for this NC subpopulation during early EB ganglion assembly and segmentation. Furthermore, restoration of wildtype chondrogenic NC in one of these mutants, prdm1a, is sufficient to restore ganglion formation, indicating a specific requirement of the chondrogenic NC for EB ganglia assembly. By contrast, analysis of the sox10 mutant, which lacks gliogenic NC, reveals that the initial assembly of ganglia is not affected. However, during later stages of development, EB ganglia are dispersed in the sox10 mutant, suggesting that glia are required to maintain normal EB ganglion morphology. These results highlight novel roles for two subpopulations of NC cells in the formation and maintenance of EB ganglia: chondrogenic NC promotes the early-stage formation of the developing EB ganglia while glial NC is required for the late-stage maintenance of ganglion morphology.

Original languageEnglish (US)
Article numbere24443
JournalPLoS One
Volume6
Issue number9
DOIs
StatePublished - Sep 9 2011

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neural crest
Neural Crest
Ganglia
Arches
Restoration
Neurons
Condensation
mutants
Neuroglia
neuroglia
Zebrafish
Danio rerio
Maintenance
Population
Branchial Region
sensory neurons
Sensory Receptor Cells
Embryonic Development
Vertebrates

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chondrogenic and gliogenic subpopulations of neural crest play distinct roles during the assembly of epibranchial ganglia. / Culbertson, Maya D.; Lewis, Zachary R.; Nechiporuk, Alex.

In: PLoS One, Vol. 6, No. 9, e24443, 09.09.2011.

Research output: Contribution to journalArticle

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