Chitosan dressing promotes healing in third degree burns in mice

Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling

Ruth M. Baxter, Tianhong Dai, Jess Kimball, Eugenia Wang, Michael R. Hamblin, William P. Wiesmann, Simon McCarthy, Shenda M. Baker

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down-regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT-PCR showed modulated mRNA levels for TGFb1 by the chitosan dressing. TGFb1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFb1 could be a powerful biomarker for future studies of the wound-healing potential of chitosan based and other treatments for burn wounds.

Original languageEnglish (US)
Pages (from-to)340-348
Number of pages9
JournalJournal of Biomedical Materials Research - Part A
Volume101 A
Issue number2
DOIs
StatePublished - Feb 2013
Externally publishedYes

Fingerprint

Tissue regeneration
Chitosan
Gene expression
Genes
Chitin
Granulation
Biomarkers
Lactic Acid
Health
Tissue
Derivatives
Messenger RNA

Keywords

  • Biomarkers
  • Chitosan
  • Expression profiling
  • TGFb1
  • Wound healing

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys

Cite this

Chitosan dressing promotes healing in third degree burns in mice : Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. / Baxter, Ruth M.; Dai, Tianhong; Kimball, Jess; Wang, Eugenia; Hamblin, Michael R.; Wiesmann, William P.; McCarthy, Simon; Baker, Shenda M.

In: Journal of Biomedical Materials Research - Part A, Vol. 101 A, No. 2, 02.2013, p. 340-348.

Research output: Contribution to journalArticle

Baxter, Ruth M. ; Dai, Tianhong ; Kimball, Jess ; Wang, Eugenia ; Hamblin, Michael R. ; Wiesmann, William P. ; McCarthy, Simon ; Baker, Shenda M. / Chitosan dressing promotes healing in third degree burns in mice : Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling. In: Journal of Biomedical Materials Research - Part A. 2013 ; Vol. 101 A, No. 2. pp. 340-348.
@article{9de2b5a298114208b3b1fc19c1905d73,
title = "Chitosan dressing promotes healing in third degree burns in mice: Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling",
abstract = "Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down-regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT-PCR showed modulated mRNA levels for TGFb1 by the chitosan dressing. TGFb1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFb1 could be a powerful biomarker for future studies of the wound-healing potential of chitosan based and other treatments for burn wounds.",
keywords = "Biomarkers, Chitosan, Expression profiling, TGFb1, Wound healing",
author = "Baxter, {Ruth M.} and Tianhong Dai and Jess Kimball and Eugenia Wang and Hamblin, {Michael R.} and Wiesmann, {William P.} and Simon McCarthy and Baker, {Shenda M.}",
year = "2013",
month = "2",
doi = "10.1002/jbm.a.34328",
language = "English (US)",
volume = "101 A",
pages = "340--348",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "1552-4973",
publisher = "Heterocorporation",
number = "2",

}

TY - JOUR

T1 - Chitosan dressing promotes healing in third degree burns in mice

T2 - Gene expression analysis shows biphasic effects for rapid tissue regeneration and decreased fibrotic signaling

AU - Baxter, Ruth M.

AU - Dai, Tianhong

AU - Kimball, Jess

AU - Wang, Eugenia

AU - Hamblin, Michael R.

AU - Wiesmann, William P.

AU - McCarthy, Simon

AU - Baker, Shenda M.

PY - 2013/2

Y1 - 2013/2

N2 - Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down-regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT-PCR showed modulated mRNA levels for TGFb1 by the chitosan dressing. TGFb1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFb1 could be a powerful biomarker for future studies of the wound-healing potential of chitosan based and other treatments for burn wounds.

AB - Burns are a significant health challenge and healing can result in scar formation. Chitosan, a derivative of chitin, has been used to promote wound healing. In this study we used gene expression profiling in a mouse model of full thickness cutaneous burn to assess the benefits of treating with a chitosan lactate dressing. Three days after wounding mice treated with chitosan showed increased expression of genes associated with formation of granulation tissue. At a later time point, seven days after wounding, genes that initially showed increased expression were now down-regulated, and there was increased expression of genes involved in remodeling suggesting that the chitosan treatment results in accelerated healing. Quantitative RT-PCR showed modulated mRNA levels for TGFb1 by the chitosan dressing. TGFb1 initially promotes healing but extended activity can result in scarring. Importantly we found that expression was elevated at day three, but decreased at day seven suggesting that chitosan treatment will not result in scar formation, and may even be beneficial in preventing scar formation. Additionally, the biphasic regulation of expression of TGFb1 could be a powerful biomarker for future studies of the wound-healing potential of chitosan based and other treatments for burn wounds.

KW - Biomarkers

KW - Chitosan

KW - Expression profiling

KW - TGFb1

KW - Wound healing

UR - http://www.scopus.com/inward/record.url?scp=84872674466&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872674466&partnerID=8YFLogxK

U2 - 10.1002/jbm.a.34328

DO - 10.1002/jbm.a.34328

M3 - Article

VL - 101 A

SP - 340

EP - 348

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 1552-4973

IS - 2

ER -