Chicken growth-associated protein (GAP)-43: primary structure and regulated expression of mRNA during embryogenesis

Lawrence Baizer, Serhan Alkan, Kate Stocker, Gary Ciment

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

Growth-associated protein (GAP)-43 is a neuron-specific phosphoprotein whose expression is associated with axonal outgrowth during neuronal development and regeneration. In order to investigate the expression of this gene product in the early developing nervous system we have isolated and sequenced a cDNA for chicken GAP-43. The predicted amino acid sequence for chicken GAP-43 displays extensive similarity to that of the mammalian protein, particularly in the amino-terminal region, to which functional domains of the protein have been assigned. The cDNA hybridizes with two RNAs of differing molecular weights on Northern blots: both appear to be regulated similarly. These RNAs first appear in the brain on embryonic day 3 (E3), suggesting that GAP-43 begins to be expressed when neuroblasts become post-mitotic. In situ hybridization analysis reveals that GAP-43 RNA is expressed by several neural structures in the chick embryo, including derivatives of the neural tube, neural crest, and neuroectodermal placodes.

Original languageEnglish (US)
Pages (from-to)61-68
Number of pages8
JournalMolecular Brain Research
Volume7
Issue number1
DOIs
StatePublished - Jan 1990

Keywords

  • Chicken embryogenesis
  • Growth-associated protein-43
  • In situ hybridization
  • Neuronal development
  • Northern blot analysis
  • cDNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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