Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

Ioanna Keklikoglou, Chiara Cianciaruso, Esra Güç, Mario Leonardo Squadrito, Laura M. Spring, Simon Tazzyman, Lore Lambein, Amanda Poissonnier, Gino B. Ferraro, Caroline Baer, Antonino Cassará, Alan Guichard, M. Luisa Iruela-Arispe, Claire E. Lewis, Lisa Coussens, Aditya Bardia, Rakesh K. Jain, Jeffrey W. Pollard, Michele De Palma

Research output: Contribution to journalArticle

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Abstract

Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental studies in mice also suggest that chemotherapy has pro-metastatic effects. Primary tumours release extracellular vesicles (EVs), including exosomes, that can facilitate the seeding and growth of metastatic cancer cells in distant organs, but the effects of chemotherapy on tumour-derived EVs remain unclear. Here we show that two classes of cytotoxic drugs broadly employed in pre-operative (neoadjuvant) breast cancer therapy, taxanes and anthracyclines, elicit tumour-derived EVs with enhanced pro-metastatic capacity. Chemotherapy-elicited EVs are enriched in annexin A6 (ANXA6), a Ca 2+ -dependent protein that promotes NF-κB-dependent endothelial cell activation, Ccl2 induction and Ly6C + CCR2 + monocyte expansion in the pulmonary pre-metastatic niche to facilitate the establishment of lung metastasis. Genetic inactivation of Anxa6 in cancer cells or Ccr2 in host cells blunts the pro-metastatic effects of chemotherapy-elicited EVs. ANXA6 is detected, and potentially enriched, in the circulating EVs of breast cancer patients undergoing neoadjuvant chemotherapy.

Original languageEnglish (US)
Pages (from-to)190-202
Number of pages13
JournalNature Cell Biology
Volume21
Issue number2
DOIs
StatePublished - Feb 1 2019

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Breast Neoplasms
Drug Therapy
Neoplasms
Annexin A6
Exosomes
Annexins
Taxoids
Lung
Anthracyclines
Extracellular Vesicles
Monocytes
Endothelial Cells
Neoplasm Metastasis
Therapeutics
Growth
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Keklikoglou, I., Cianciaruso, C., Güç, E., Squadrito, M. L., Spring, L. M., Tazzyman, S., ... De Palma, M. (2019). Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nature Cell Biology, 21(2), 190-202. https://doi.org/10.1038/s41556-018-0256-3

Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. / Keklikoglou, Ioanna; Cianciaruso, Chiara; Güç, Esra; Squadrito, Mario Leonardo; Spring, Laura M.; Tazzyman, Simon; Lambein, Lore; Poissonnier, Amanda; Ferraro, Gino B.; Baer, Caroline; Cassará, Antonino; Guichard, Alan; Iruela-Arispe, M. Luisa; Lewis, Claire E.; Coussens, Lisa; Bardia, Aditya; Jain, Rakesh K.; Pollard, Jeffrey W.; De Palma, Michele.

In: Nature Cell Biology, Vol. 21, No. 2, 01.02.2019, p. 190-202.

Research output: Contribution to journalArticle

Keklikoglou, I, Cianciaruso, C, Güç, E, Squadrito, ML, Spring, LM, Tazzyman, S, Lambein, L, Poissonnier, A, Ferraro, GB, Baer, C, Cassará, A, Guichard, A, Iruela-Arispe, ML, Lewis, CE, Coussens, L, Bardia, A, Jain, RK, Pollard, JW & De Palma, M 2019, 'Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models' Nature Cell Biology, vol. 21, no. 2, pp. 190-202. https://doi.org/10.1038/s41556-018-0256-3
Keklikoglou I, Cianciaruso C, Güç E, Squadrito ML, Spring LM, Tazzyman S et al. Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nature Cell Biology. 2019 Feb 1;21(2):190-202. https://doi.org/10.1038/s41556-018-0256-3
Keklikoglou, Ioanna ; Cianciaruso, Chiara ; Güç, Esra ; Squadrito, Mario Leonardo ; Spring, Laura M. ; Tazzyman, Simon ; Lambein, Lore ; Poissonnier, Amanda ; Ferraro, Gino B. ; Baer, Caroline ; Cassará, Antonino ; Guichard, Alan ; Iruela-Arispe, M. Luisa ; Lewis, Claire E. ; Coussens, Lisa ; Bardia, Aditya ; Jain, Rakesh K. ; Pollard, Jeffrey W. ; De Palma, Michele. / Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. In: Nature Cell Biology. 2019 ; Vol. 21, No. 2. pp. 190-202.
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