Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

Ioanna Keklikoglou; Chiara Cianciaruso; Esra Güç; Mario Leonardo Squadrito; Laura M. Spring; Simon Tazzyman; Lore Lambein; Amanda Poissonnier; Gino B. Ferraro; Caroline Baer; Antonino Cassará; Alan Guichard; M. Luisa Iruela-Arispe; Claire E. Lewis; Lisa M. Coussens; Aditya Bardia; Rakesh K. Jain; Jeffrey W. Pollard; Michele De Palma

doi:10.1038/s41556-018-0256-3

Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

Ioanna Keklikoglou, Chiara Cianciaruso, Esra Güç, Mario Leonardo Squadrito, Laura M. Spring, Simon Tazzyman, Lore Lambein, Amanda Poissonnier, Gino B. Ferraro, Caroline Baer, Antonino Cassará, Alan Guichard, M. Luisa Iruela-Arispe, Claire E. Lewis, Lisa M. Coussens, Aditya Bardia, Rakesh K. Jain, Jeffrey W. Pollard, Michele De Palma

Cell Developmental and Cancer Biology

Research output: Contribution to journal › Article › peer-review

363 Scopus citations

Abstract

Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental studies in mice also suggest that chemotherapy has pro-metastatic effects. Primary tumours release extracellular vesicles (EVs), including exosomes, that can facilitate the seeding and growth of metastatic cancer cells in distant organs, but the effects of chemotherapy on tumour-derived EVs remain unclear. Here we show that two classes of cytotoxic drugs broadly employed in pre-operative (neoadjuvant) breast cancer therapy, taxanes and anthracyclines, elicit tumour-derived EVs with enhanced pro-metastatic capacity. Chemotherapy-elicited EVs are enriched in annexin A6 (ANXA6), a Ca ²⁺ -dependent protein that promotes NF-κB-dependent endothelial cell activation, Ccl2 induction and Ly6C ⁺ CCR2 ⁺ monocyte expansion in the pulmonary pre-metastatic niche to facilitate the establishment of lung metastasis. Genetic inactivation of Anxa6 in cancer cells or Ccr2 in host cells blunts the pro-metastatic effects of chemotherapy-elicited EVs. ANXA6 is detected, and potentially enriched, in the circulating EVs of breast cancer patients undergoing neoadjuvant chemotherapy.

Original language	English (US)
Pages (from-to)	190-202
Number of pages	13
Journal	Nature Cell Biology
Volume	21
Issue number	2
DOIs	https://doi.org/10.1038/s41556-018-0256-3
State	Published - Feb 1 2019

ASJC Scopus subject areas

Cell Biology

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Keklikoglou, I., Cianciaruso, C., Güç, E., Squadrito, M. L., Spring, L. M., Tazzyman, S., Lambein, L., Poissonnier, A., Ferraro, G. B., Baer, C., Cassará, A., Guichard, A., Iruela-Arispe, M. L., Lewis, C. E., Coussens, L. M., Bardia, A., Jain, R. K., Pollard, J. W., & De Palma, M. (2019). Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models. Nature Cell Biology, 21(2), 190-202. https://doi.org/10.1038/s41556-018-0256-3

Keklikoglou, I, Cianciaruso, C, Güç, E, Squadrito, ML, Spring, LM, Tazzyman, S, Lambein, L, Poissonnier, A, Ferraro, GB, Baer, C, Cassará, A, Guichard, A, Iruela-Arispe, ML, Lewis, CE, Coussens, LM, Bardia, A, Jain, RK, Pollard, JW & De Palma, M 2019, 'Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models', Nature Cell Biology, vol. 21, no. 2, pp. 190-202. https://doi.org/10.1038/s41556-018-0256-3

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abstract = " Cytotoxic chemotherapy is an effective treatment for invasive breast cancer. However, experimental studies in mice also suggest that chemotherapy has pro-metastatic effects. Primary tumours release extracellular vesicles (EVs), including exosomes, that can facilitate the seeding and growth of metastatic cancer cells in distant organs, but the effects of chemotherapy on tumour-derived EVs remain unclear. Here we show that two classes of cytotoxic drugs broadly employed in pre-operative (neoadjuvant) breast cancer therapy, taxanes and anthracyclines, elicit tumour-derived EVs with enhanced pro-metastatic capacity. Chemotherapy-elicited EVs are enriched in annexin A6 (ANXA6), a Ca 2+ -dependent protein that promotes NF-κB-dependent endothelial cell activation, Ccl2 induction and Ly6C + CCR2 + monocyte expansion in the pulmonary pre-metastatic niche to facilitate the establishment of lung metastasis. Genetic inactivation of Anxa6 in cancer cells or Ccr2 in host cells blunts the pro-metastatic effects of chemotherapy-elicited EVs. ANXA6 is detected, and potentially enriched, in the circulating EVs of breast cancer patients undergoing neoadjuvant chemotherapy.",

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AU - Cassará, Antonino

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AU - Iruela-Arispe, M. Luisa

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AU - Coussens, Lisa M.

AU - Bardia, Aditya

AU - Jain, Rakesh K.

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AU - De Palma, Michele

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Chemotherapy elicits pro-metastatic extracellular vesicles in breast cancer models

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