Chemotactic Activity of Lens Proteins and the Pathogenesis Of Phacolytic Glaucoma

James (Jim) Rosenbaum, John R. Samples, Brian Seymour, Lisa Langlois, Larry David

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Leakage of lens proteins from a hypermature cataract can result in a characteristic glaucoma that is associated with the invasion of the anterior chamber by monocytes. We hypothesized that the lens proteins themselves might account for the monocyte response. A sonicated lens induced concentration-dependent migration of monocytes in a Boyden chamber assay system. Checkerboard analysis indicated that the movement was directed rather than merely random. Relative to a control chemoattractant, N-formyl-methionyl-leucyl-phenylalanine, the lens induced monocyte migration more potently than neutrophil migration. The ability to induce migration was markedly reduced by incubating the lens with either trypsin or papain. Chemotactic activity was readily demonstrable in lenses from young donors without cataracts. Separation of lens proteins by gel filtration with high-performance liquid chromatography indicated that the chemotactic activity was most consistently associated with the gamma crystallin fraction. The chemotactic activity of lens proteins may contribute to the pathogenesis of phacolytic glaucoma or the uveitis resulting from retained cortical material after cataract extraction.

Original languageEnglish (US)
Pages (from-to)1582-1584
Number of pages3
JournalArchives of Ophthalmology
Volume105
Issue number11
DOIs
StatePublished - 1987

Fingerprint

Crystallins
Glaucoma
Lenses
Monocytes
Cataract
gamma-Crystallins
N-Formylmethionine Leucyl-Phenylalanine
Papain
Cataract Extraction
Chemotactic Factors
Uveitis
Anterior Chamber
Trypsin
Gel Chromatography
Neutrophils
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Chemotactic Activity of Lens Proteins and the Pathogenesis Of Phacolytic Glaucoma. / Rosenbaum, James (Jim); Samples, John R.; Seymour, Brian; Langlois, Lisa; David, Larry.

In: Archives of Ophthalmology, Vol. 105, No. 11, 1987, p. 1582-1584.

Research output: Contribution to journalArticle

Rosenbaum, James (Jim) ; Samples, John R. ; Seymour, Brian ; Langlois, Lisa ; David, Larry. / Chemotactic Activity of Lens Proteins and the Pathogenesis Of Phacolytic Glaucoma. In: Archives of Ophthalmology. 1987 ; Vol. 105, No. 11. pp. 1582-1584.
@article{f642accf196c4f7ab89b8ca985e498da,
title = "Chemotactic Activity of Lens Proteins and the Pathogenesis Of Phacolytic Glaucoma",
abstract = "Leakage of lens proteins from a hypermature cataract can result in a characteristic glaucoma that is associated with the invasion of the anterior chamber by monocytes. We hypothesized that the lens proteins themselves might account for the monocyte response. A sonicated lens induced concentration-dependent migration of monocytes in a Boyden chamber assay system. Checkerboard analysis indicated that the movement was directed rather than merely random. Relative to a control chemoattractant, N-formyl-methionyl-leucyl-phenylalanine, the lens induced monocyte migration more potently than neutrophil migration. The ability to induce migration was markedly reduced by incubating the lens with either trypsin or papain. Chemotactic activity was readily demonstrable in lenses from young donors without cataracts. Separation of lens proteins by gel filtration with high-performance liquid chromatography indicated that the chemotactic activity was most consistently associated with the gamma crystallin fraction. The chemotactic activity of lens proteins may contribute to the pathogenesis of phacolytic glaucoma or the uveitis resulting from retained cortical material after cataract extraction.",
author = "Rosenbaum, {James (Jim)} and Samples, {John R.} and Brian Seymour and Lisa Langlois and Larry David",
year = "1987",
doi = "10.1001/archopht.1987.01060110128046",
language = "English (US)",
volume = "105",
pages = "1582--1584",
journal = "JAMA Ophthalmology",
issn = "2168-6165",
publisher = "American Medical Association",
number = "11",

}

TY - JOUR

T1 - Chemotactic Activity of Lens Proteins and the Pathogenesis Of Phacolytic Glaucoma

AU - Rosenbaum, James (Jim)

AU - Samples, John R.

AU - Seymour, Brian

AU - Langlois, Lisa

AU - David, Larry

PY - 1987

Y1 - 1987

N2 - Leakage of lens proteins from a hypermature cataract can result in a characteristic glaucoma that is associated with the invasion of the anterior chamber by monocytes. We hypothesized that the lens proteins themselves might account for the monocyte response. A sonicated lens induced concentration-dependent migration of monocytes in a Boyden chamber assay system. Checkerboard analysis indicated that the movement was directed rather than merely random. Relative to a control chemoattractant, N-formyl-methionyl-leucyl-phenylalanine, the lens induced monocyte migration more potently than neutrophil migration. The ability to induce migration was markedly reduced by incubating the lens with either trypsin or papain. Chemotactic activity was readily demonstrable in lenses from young donors without cataracts. Separation of lens proteins by gel filtration with high-performance liquid chromatography indicated that the chemotactic activity was most consistently associated with the gamma crystallin fraction. The chemotactic activity of lens proteins may contribute to the pathogenesis of phacolytic glaucoma or the uveitis resulting from retained cortical material after cataract extraction.

AB - Leakage of lens proteins from a hypermature cataract can result in a characteristic glaucoma that is associated with the invasion of the anterior chamber by monocytes. We hypothesized that the lens proteins themselves might account for the monocyte response. A sonicated lens induced concentration-dependent migration of monocytes in a Boyden chamber assay system. Checkerboard analysis indicated that the movement was directed rather than merely random. Relative to a control chemoattractant, N-formyl-methionyl-leucyl-phenylalanine, the lens induced monocyte migration more potently than neutrophil migration. The ability to induce migration was markedly reduced by incubating the lens with either trypsin or papain. Chemotactic activity was readily demonstrable in lenses from young donors without cataracts. Separation of lens proteins by gel filtration with high-performance liquid chromatography indicated that the chemotactic activity was most consistently associated with the gamma crystallin fraction. The chemotactic activity of lens proteins may contribute to the pathogenesis of phacolytic glaucoma or the uveitis resulting from retained cortical material after cataract extraction.

UR - http://www.scopus.com/inward/record.url?scp=0023231253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023231253&partnerID=8YFLogxK

U2 - 10.1001/archopht.1987.01060110128046

DO - 10.1001/archopht.1987.01060110128046

M3 - Article

C2 - 3675292

AN - SCOPUS:0023231253

VL - 105

SP - 1582

EP - 1584

JO - JAMA Ophthalmology

JF - JAMA Ophthalmology

SN - 2168-6165

IS - 11

ER -